| 吴瑞，朱家红，梁黔云.巴西橡胶树HbMT2植物表达载体的构建及遗传转化烟草[J].广东农业科学,2013,40(6):138-142 |
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巴西橡胶树HbMT2植物表达载体的构建及遗传转化烟草 |
| Construction of plant expression vector for the HbMT2 gene of Hevea brasiliensis and its genetic transformation tobacco |
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| DOI： |
| 中文关键词: 巴西橡胶树 金属硫蛋白 载体构建 遗传转化 抗逆性分析 |
| 英文关键词: Hevea brasiliensis metallothionein(MT) construction of expression genetic transformation resistance analysis |
| 基金项目:天然橡胶产业技术体系基金（CARS-34-ZD1）；国家自然科学基金（30760917） |
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| 中文摘要: |
| 将巴西橡胶树金属硫蛋白基因HbMT2 插入到植物表达载体pCAMBIA1304，得到HbMT2 基因的植物表达载体pCAMBIA1304-HbMT2。通过电击法将该载体导入根癌农杆菌菌株中并转化烟草。将获得的具有潮霉素(Hyg)抗性的再生植株进行PCR 鉴定，结果表明目的基因已经整合到烟草基因组中。抗逆性分析试验显示，转基因烟草对H2O2处理和紫外线照射耐受性显著提高；重金属离子胁迫下，转基因烟草叶绿素含量、过氧化物酶和过氧化氢酶活性明显高于对照。 |
| 英文摘要: |
| The HbMT2 was constructed into pCAMBIA1304 expression vector, and transformed into tobacco (Nicotiana tabacum)
mediated by Agrobacterium, Hygromycin B (Hyg) resistant regenerated plantlets of tobacco were selected. PCR detection indicated that all the Hyg resistant tobacco plants contained the alien HbMT2 gene. Resistance analysis tests showed that the tolerance of transgenic plants to hydrogen peroxide and ultraviolet irradiation was significantly increased, and the chlorophyll content and the activity of peroxidase and catalase were significantly higher in trangenic plants than the controls in heavy-metal stress condition. |
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