| 周丹华，孙大元，王慧，陈志强,王国宝，余国新.空间诱变水稻H4成熟胚再生体系的建立[J].广东农业科学,2013,40(7):1-3 |
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空间诱变水稻H4成熟胚再生体系的建立 |
| Study on the regeneration system from mature embryo of the indica rice H4 |
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| DOI： |
| 中文关键词: 空间诱变 成熟胚 组织培养 |
| 英文关键词: space mutation mature embryo tissue culture |
| 基金项目:国家“863”计划项目（2011AA10A101）；广东省科技计划项目（2011A020102004）；国家现代农业产业技术体系建设专项（CARS-01-12）；广东省农业科技研究团队项目（20110203） |
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| 中文摘要: |
| 以空间诱变水稻H4成熟胚为外植体，通过调节2袁4-D浓度、激素组合和分化途径，建立适宜的再生体系。结果表明：诱导培养基以NMB为基本培养基，添加2.5 mg/L2，4-D、1.0 mg/LNAA和0.5 mg/L 6-BA，26℃暗培养30d，愈伤诱导率达91.63%；继代培养基中添加2mg/LABA，对胚性愈伤组织的形成及后续分化率有一定的促进作用；两步分化法可提高愈伤组织的分化效率，分化率达84.76%，植株再生率为77.67%。初步建立了H4成熟胚愈伤组织的再生体系，为后续的遗传转化研究奠定基础。 |
| 英文摘要: |
| To establish a suitable regeneration system of space-induced indica rice, H4, a series of experimental programs were carried out. The results showed that When NMB was used as the basic medium, 2.5 mg/L 2,4-D, 1.0 mg/L NAA and 0.5 mg/L 6-BA were added, the callus induction frequency of H4 was 91.63%; ABA showed a significant result on the growth and regeneration of callus; the two -step culture method exhibited positive effects on enhancing plant regeneration ability. The regeneration technology for high frequency of H4 mature embryos was established preliminarily. |
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