| 孙静贤，高玉红，杨海英，张广求，孙云龙，杜刚.滇重楼内生真菌抗菌活性的筛选和菌株鉴定[J].广东农业科学,2013,40(9):74-76 |
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滇重楼内生真菌抗菌活性的筛选和菌株鉴定 |
| Antibacterial activity screening and identification of endophytic fungi from Paris polyphylla Smith var. yunnanensis (Franch.) Hand. -Mazz |
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| DOI： |
| 中文关键词: 滇重楼 内生真菌 抑菌活性 分子鉴定 |
| 英文关键词: Paris polyphylla Smith var. yunnanensis (Franch.) Hand.-Mazz endophytic fungi antimicrobial activity molecular identification |
| 基金项目:云南省科技联合专项(2011FB124)；云南省教育厅科学研究基金重点项目（2011Z005） |
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| 中文摘要: |
| 以金黄色葡萄球菌（Staphylococcus aureus）、大肠杆菌(Escherichia coli)和产气肠杆菌(Enterobacter aerogenes)为指示菌种，采用滤纸片法对分离自滇重楼也Paris polyphylla Smith var. yunnanensis (Franch.) Hand. -Mazz.页的10 株内生真菌进行抗菌活性检测。结果发现，10株被测菌株的发酵物乙醇提取物中有3株无抗菌活性，另外7株均只对金黄色葡萄球菌有抑制作用。其中菌株YL0401发酵物乙醇提取物具有较高的抗菌活性袁抑菌圈直径为30.7mm，抑菌效果为72.7%。结合形态学特征和ITS-rDNA序列分析确定菌株YL0401为墙粘鞭霉（Gliomastix murorum冤遥 |
| 英文摘要: |
| The antimicrobial activity of 10 endophytic fungi isolated from traditional Chinese medicinal plant Paris polyphylla Smith var. yunnanensis (Franch.) Hand. -Mazz. were detected by the method of Filter method test with Staphylococcus aureus, Escherichia coli and Enterobacter aerogenes as indicator microorganisms. The results showed that the ethanol extracts of three isolated entophytic fungi strains had no antibiotic activity, while the other 7 strains had antibiotic activity against S. aureus. Especially the stain YL0401 obviously exhibited strongest antibiotic activity against S. aureus, the inhibition zones diameter was 30.7 mm and the antibiotic effect was 72.7%.Based on the morphological characters and ITS-rDNA sequence analysis袁the endophytic fungi strain YL0401 was identified as Gliomastix murorum |
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