王鹏飞,马养民,孔阳.猫儿屎内生真菌分离鉴定及抑菌活性研究[J].广东农业科学,2013,40(13):85-87 |
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猫儿屎内生真菌分离鉴定及抑菌活性研究 |
Isolation, identification and antimicrobial activities of endophytic fungi from Decaisnea fargesii Franch. |
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DOI: |
中文关键词: 猫儿屎 内生真菌 抑菌活性 |
英文关键词: Decaisnea fargesii Franch. Endophytic Fungi atibacterial ativity |
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中文摘要: |
以新鲜猫儿屎为材料,采用组织分离法从其茎、叶、果实中分离内生真菌,采用形态学方法进行初步鉴定;采用滤纸片法测定猫儿屎内生真菌次生代谢产物的抑菌活性。从猫儿屎的茎、叶和果实中共分离出91 株内生真菌,分别属于6目7科15 属;曲霉属为优势种属,占总量的32%;91 株内生真菌具有不同程度的抑菌活性,菌种DS37、DS58、DL06 和DL14 的抑菌活性较强。结果表明,猫儿屎内生真菌种类具有多样性,次生代谢产物具有广泛的抑菌活性。 |
英文摘要: |
Endophytic fungi were isolated from the stem, leaf and fruit of Decaisnea fargesii Franch., and then morphologically identified; the antimicrobial activities were screened by paper sheet method. 91 strains of endophytic fungi were isolated from Decaisnea fargesii Franch., they were identified as 6 orders, 7 families, 15 genus. Aspergillus was the dominant species, accounting for 32 % of the total. Almost 91 strains presented antimicrobial activities, DS37, DS58, DL06 and DL14 has a strong antimicrobial activity. Endophytic fungi exist in Decaisnea fargesii Franch. have a variety of species, their secondary metabolites with a wide range of antimicrobial activity. |
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