| 姚东校，洪鲲，杨立昌，乙引，万晴姣.马铃薯X病毒贵州分离物的分子鉴定[J].广东农业科学,2013,40(13):139-141 |
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马铃薯X病毒贵州分离物的分子鉴定 |
| Molecular identification of a potato virus X isolated from potatos in Guizhou |
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| DOI： |
| 中文关键词: 马铃薯X病毒 外壳蛋白 亲缘分析 |
| 英文关键词: potato virus X coat protein phylogenetic analysis |
| 基金项目: |
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| 中文摘要: |
| 从贵州威宁县染病马铃薯植株中分离得到马铃薯X病毒贵州分离物（PVX-GZ），采用RT-PCR扩增并克隆了该分离物的外壳蛋白基因（CP）。经测序，该CP基因长714 bp，编码237个氨基酸残基，分子量25.2 ku；与19种已报道的PVX各地分离物的同源性相比，其核苷酸和氨基酸序列分别在78.6%~97.5%和84.8%~99.6%之间，其中，与两种典型的PVX X3株系（荷兰分离物X3和英国分离物UK3）的基因序列同源性达97.1%以上。结合寄主鉴别结果，判断该分离物属PVX的X3株系。 |
| 英文摘要: |
| The coat protein (CP) gene of a potato virus X (PVX-GZ) isolated from potato in Guizhou was cloned and sequenced. The CP gene was 714 bp long and encoded a 237 amino acid residues with a predicted molecular mass of 25.2 ku. Sequence comparison results showed that the nucleotide and deduced amino acids sequences of the gene share homology with 19 reported PVX isolates were 78.6%~97.5% and 84.8%~99.6%, respectively. The highest nucleotide sequence homology (over 97.1%) was reached when compared with the isolated X3 from Holland and UK3 from UK, two representative isolates of PVX strain X3. Consistent results from differential host test revealed that PVX-GZ belonged to strain X3. |
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