| 李紫聪，石俊松，罗绿花，周荣，周秀，麦然标，吴珍芳.氧分压对猪卵母细胞成熟尧受精及早期胚胎发育的影响[J].广东农业科学,2013,40(15):145-148 |
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氧分压对猪卵母细胞成熟尧受精及早期胚胎发育的影响 |
| Effect of oxygen concentration on porcine oocyte maturation,in vitro fertilization and early embryo development |
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| DOI： |
| 中文关键词: 卵母细胞 胚胎发育 猪 |
| 英文关键词: oocytes embryo development porcine |
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| 摘要点击次数: 1599 |
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| 中文摘要: |
| 研究利用两种氧分压（5%和20%）培养卵母细胞并进行IVF试验，同时使用两种氧分压培养孤雌（PA）胚胎、体外受精（IVF）胚胎和克隆(NT) 胚胎，以探寻猪的最佳体外培养环境。结果显示：低氧分压不利于猪卵母细胞的体外成熟，其成熟率极显著低于高氧培养的，但对低氧培养的卵母细胞进行IVF时，其卵裂率和囊胚率均极显著高于高氧培养的卵母细胞；IVF进行精卵孵育时，低氧环境下，其胚胎卵裂率和囊胚率均显著高于高氧环境下的；PA胚胎、IVF胚胎和NT胚胎用两种氧分压培养，低氧条件下IVF胚胎的发育能力高于高氧培养的，而PA胚胎和NT胚胎差异不显著。试验结果表明，低氧培养虽不利于卵母细胞的成熟，但有利于其以后的胚胎发育，且低氧环境下IVF胚胎的发育能力高于高氧环境下的。 |
| 英文摘要: |
| In this study, the effect of atmospheric (20%) and reduced (5%) level of O2 on porcine oocyte maturation, in vitro
fertilization and early embryo development was investigated. The results showed that: reduced O2 concentration results in a lower oocyte
maturation rate than that associated with atmospheric O2 level. However, IVF embryos derived from oocytes cultured with 5% of O2 had a
higher cleavage rate and blastocyst rate, compared to IVF embryos generated from oocytes cultured under atmospheric level of O2. In vitro fertilization that was performed under reduced O2 level results in a higher cleavage rate and blastocyst rate of IVF embryos, compared to that associated with in vitro fertilization performed under atmospheric O2 level. No significant difference in the developmental rate of PA embryos and SCNT embryos was found between 20% and 5% O2 culture condition. However, IVF embryos cultured with 5% of O2 have a higher developmental capacity, compared to IVF embryos cultured with 20% of O2. These results suggested that reduced O2 concentration inhibited oocyte maturation but increased its in vitro fertilization rate, and reduced O2 concentration increased the developmental rate of IVF embryos. |
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