陈云云,罗静瑶,赵惠,穆雷,谢俊,韦双双,夏幽泉,汤华.番茄果实颜色相关MYB转录因子的基因表达研究[J].广东农业科学,2013,40(18):127-129 |
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番茄果实颜色相关MYB转录因子的基因表达研究 |
Research on gene expression of MYB transcription factor related to tomato fruit color |
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DOI: |
中文关键词: 番茄 果实颜色 黄果突变体 MYB转录因子 基因表达 |
英文关键词: tomato fruit color yellow-fruit mutant MYB transcription factor gene expression |
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中文摘要: |
在番茄果实颜色发育过程中,转录因子参与其中。在源于番茄野生种Solanum lycopersicoides的导入系群体中,发现了1个黄果突变体,以红果亲本番茄及该黄果突变体为遗传材料,通过数字基因表达谱测序及RT-PCR,对MYB转录因子的表达模式进行研究。筛选到多个与果实颜色相关的差异表达MYB转录因子,并进行了半定量RT-PCR验证。 |
英文摘要: |
During the tomato fruit color development, transcription factor may play important roles. From an introgression line population derived from tomato wild species Solanum lycopersicoides, we found a yellow-fruit mutant line which was different from redfruit patent. We took red-fruit parent tomato and yellow-fruit mutant as genetic materials to study the gene expression profiles of MYB
transcription factor by digital gene expression sequencing and RT-PCR. As results, we screened out many MYB transcription factors related to tomato fruit color and differential expressed in yellow-fruit and red-fruit tomato, and verified one of them by RT-PCR. |
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