| 何慧怡，樊丽娜，陈勇生，徐苑娴，劳方业，齐永文，邓海华.粤糖03-393茎尖诱导丛芽培养基筛选试验[J].广东农业科学,2013,40(23):9-11 |
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粤糖03-393茎尖诱导丛芽培养基筛选试验 |
| Experiment in medium screening for tufted-bud induction from apical meristem of Yuetang 03-393 |
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| DOI： |
| 中文关键词: 甘蔗 茎尖诱导 组织培养 正交试验 |
| 英文关键词: sugarcane apical meristem tissue culture orthogonal experiment |
| 基金项目:国家现代农业产业技术体系建设专项(CARS-20-1-4)；广东省科技计划项目（2012B020301008，2012A090300015） |
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| 中文摘要: |
| 应用正交设计法研究6-苄氨基腺嘌呤（6-BA）、激动素（KT）和噻苯隆（TDZ）3种植物生长调节剂对粤糖03-393茎尖分生组织诱导丛芽生长的影响。结果表明，3种因子对茎尖诱导丛芽成苗率的影响主次顺序为6-BA>TDZ>KT，最佳浓度组合为6-BA1.50mg/L、KT0.50 mg/L、TDZ 0.03 mg/L，但这3因子对成苗率影响的差异未达显著水平。从诱导出丛芽的壮苗率看，当6-BA浓度为1.00 mg/L时其茎尖诱导丛芽的壮苗率显著高于1.50mg/L和0.50mg/ 水平，而后两个水平之间无显著差异。 |
| 英文摘要: |
| In this study, the effect of 6-benzylaminopurine(6-BA), kinetin(KT), thidiazuron(TDZ) in medium for tuftedudinduction from apical meristem of Yuetang 03-393 was analyzed by using orthogonal experiment design. The rate both of tufted-bud and of strengthen seedling after 30 days cultivation was respectively investigated. By means of visual analysis, the results indicated that the most important factor for the tufted-bud induction was 6-BA, followed by TDZ and KT; And the optimum consistency combination of three plant regulators in tufted-bud induction was 6-BA 1.50 mg/L袁KT 0.50 mg/L，TDZ 0.03 mg/L. But there was no significant difference among these three factors by the analysis of variance. What's more, when 6-BA is in 1.00 mg/L, the strengthen seedling rate is significantly higher than the rate both in 1.50 mg/L and in 0.50 mg/L. |
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