| 周伟，黄进勇.玉米农田土壤细菌群落变化的DGGE分析[J].广东农业科学,2013,40(23):43-46 |
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玉米农田土壤细菌群落变化的DGGE分析 |
| Analysis of the soil bacterial community diversity in the maize field based on PCR-DGGE |
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| DOI： |
| 中文关键词: DGGE 玉米农田土壤 细菌群落 多样性 PCR |
| 英文关键词: maize field DGGE bacterial community biodiversity PCR |
| 基金项目:/ |
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| 摘要点击次数: 1258 |
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| 中文摘要: |
| 利用改进的土壤DNA提取方法直接从玉米农田土壤样品中提取微生物全基因组，以嵌套式PCR和降落式PCR扩增出细菌16S rDNAV3区基因，并运用DGGE电泳技术对扩增片段进行分离，对玉米生长过程中4个重要生育期及不同土层深度细菌群落的变化进行了分析。结果表明，改进的提取方法能够获得完整且含量较高的基因组DNA，嵌套式PCR有效扩增了16SrDNAV3基因。随着玉米的生长以及土层深度的改变，土壤中的细菌在保持优势种群基本稳定的同时表现出丰富的多样性。其中，在苗期和灌浆期细菌群落的变化最为明显，细菌种类最丰富，而收获期细菌种类最少。同一生育期不同土层深度的细菌也存较大差异，表层土壤的细菌变化最大。 |
| 英文摘要: |
| Improved soil DNA extraction method was used to extract the whole microbial genome directly from the corn farmland soil samples, the diversity of bacterial community was studied by amplifying the bacterial 16S rDNAV3 fragment with nested and touch-down PCR methods, and separating the products by denaturing gradient gel electrophoresis (DGGE). Soil samples were taken at three different depths within four different stages. The result shown that the improved extraction method and PCR strategies was efficient, the diversity of soil bacteria was higher in maize fields. With the change of the maize growth and soil depth, the bacterial populations shown highly diverse in species but the dominants species community were stable. The most obvious community changed in the bacterial community in the period of maize seedlings and filling stage, with the most abundant species of bacteria during this period. The least types of bacteria were isolated after the harvest. There was an obviously different of soil bacteria community under different depths in the same growth period of maize 渊the soil bacteria distribution under different depths were different in the same growth period of
maize冤袁and the number of bacteria community in top soil changed more greatly than others. |
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