| 宋晓兵，彭埃天，陈霞，程保平，凌金锋.柑橘黄龙病病原培养及分子检测技术研究进展[J].广东农业科学,2013,40(23):65-69 |
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柑橘黄龙病病原培养及分子检测技术研究进展 |
| Research advances of Citrus Huanglongbing pathogen culture and detection technology |
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| DOI： |
| 中文关键词: 柑橘黄龙病 韧皮部杆菌属细菌 病原培养 检测技术 |
| 英文关键词: Huanglongbing Candidatus Liberobacter sp. pathogen isolation detection technology |
| 基金项目:广东省农业科技重大技术研究项目（201201127）广东省现代农业产业技术体系建设专项(粤财农[2009]380号)；广东省科技计划重点项目（2012A020200016） |
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| 中文摘要: |
| 柑橘黄龙病（Huanglongbing）由韧皮部杆菌属细菌（Candidatus Liberobacter sp.）侵染引起，是柑橘生产上的毁灭性病害，当前正在世界柑橘产区迅速蔓延，已成为制约柑橘产业健康发展的关键因素之一。介绍了柑橘黄龙病的症状以及病原的分离培养研究进展，阐述了病原的分子检测技术，包括PCR、双重PCR、实时荧光定量PCR和LAMP快速检测技术，并对病害的防控进行展望。 |
| 英文摘要: |
| Citrus greening disease (Huanglongbing) caused by bacterial (Candidatus Liberobacter sp.), is a devastating disease of citrus production, is currently spreading rapidly in citrus producing areas of the world and has become a key constraint factor of the citrus industry. This article describes the symptoms of citrus Huanglongbing disease and pathogen isolation and culture, a detailed molecular pathogen detection technology, including PCR, double-PCR, real-time PCR and LAMP rapid detection technology, and made the prospect of disease prevention and control. |
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