| 马勇，刘嘉荣，巴德仁贵，哈斯阿古拉.甜瓜14-3-3基因家族全基因组鉴定与进化分析[J].广东农业科学,2013,40(24):104-111 |
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甜瓜14-3-3基因家族全基因组鉴定与进化分析 |
| Genome-wide identification and phylogenetic analysis of14-3-3 gene family in melon |
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| DOI： |
| 中文关键词: 甜瓜 14-3-3蛋白 基因家族 进化分析 |
| 英文关键词: melon 14-3-3 protein gene family phylogenetic analysis |
| 基金项目:国家自然科学基金(31360486)；内蒙古大学提升综合实力重点项目(1402020201058)；包头师范学院青年科学研究基金(BSYKJ2013-29) |
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| 中文摘要: |
| 以拟南芥的14-3-3蛋白保守结构域为探针序列，对甜瓜全基因组的蛋白质数据库进行搜索，获得9个14-3-3蛋白序列，其中ε类5个、非ε类4个。对甜瓜14-3-3基因家族进行了系统发生分析、内含子数量分析、序列相似性比对，通过对所有甜瓜的14-3-3蛋白相匹配的Unigene分析后发现，部分基因在多种组织中均有表达，部分基因在甜瓜果实以及花中高丰度特异表达，而且不同成员之间的表达模式存在较大差异。甜瓜14-3-3基因家族成员的分子进化及表达模式的研究结果表明基因重复后发生功能分化。 |
| 英文摘要: |
| Nine 14-3-3 genes including five -type 14-3-3 gene, and four non-ε-type 14-3-3 family gene, were identified from the melon genome protein database which was based on conserved domains of 14-3-3 proteins as probe sequences in Arabidopsis. Phylogenetic analysis, numbers of intron analysis, alignment of sequence similarity was performed in 14-3-3 gene family in melon. The results of analysis of Unigenes matched with 14-3-3 proteins showed that 14-3-3 genes expressed in different tissues, part of genes specifically expressed with high abundance in fruit and flower,and different members of 14-3-3 gene family exhibited difference in patterns of expression. In conclusion, the results indicated that there were functional divergences in melon 14-3-3 gene family after gene duplication. |
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