| 陆燕元1 马焕成1 邓西平2.严重土壤干旱后复水条件下转基因
甘薯的光合响应特性[J].广东农业科学,2014,41(2):9-13 |
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严重土壤干旱后复水条件下转基因
甘薯的光合响应特性 |
| Photosynthetic characteristics of rewetted transgenic sweetpotato after severe drought condition and rewetted |
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| DOI： |
| 中文关键词: 转基因 甘薯 干旱 复水 光合响应曲线 |
| 英文关键词: transgenic gene sweet potato drought rewetted photosynthetic response curves |
| 基金项目:国家自然科学基金31260175 |
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| 摘要点击次数: 1516 |
| 全文下载次数: 636 |
| 中文摘要: |
| 采用盆栽试验在重度干旱胁迫（土壤田间持水量的35%~40%及复水条件下,对同时转入Cu/Zn SOD
和APX 基因的甘薯和未转基因对照植株的光合响应特性进行研究结果表明,干旱胁迫及复水过程中转基因和未
转基因植株光响应曲线的变化趋势基本一致采用非直角双曲线模型拟合良好转基因TS和未转基因甘薯NS的
最大净光合速率Pmax光饱和点LSP和表观量子效率（渍暗呼吸速率（Rd）在干旱胁迫下均明显降低复水后又逐
渐增加但与NS 植株相比TS 植株在胁迫条件下降低的幅度较小，而在复水过程中恢复得也更快,反映出转入的抗
氧化酶基因提高了甘薯在干旱胁迫条件下的光合能力及修复能力,增强了对干旱胁迫的适应性TS 和NS 植株的光
补偿点（LCP）在干旱胁迫下显著提高,但TS 植株上升的幅度比NS 植株小复水过程中修复得也更快,表明转基因甘
薯增强了对光环境的适应性. |
| 英文摘要: |
| A comparative study on photosynthetic characteristics between the genetic modified sweet potato transferred
with the Cu/Zn SOD and APX genes (TS) and non-GM sweet potato (NS) was carried out with pot experiments. Three
controls, namely the favorable soil moisture (75%~80% of field water capacity, FWC), the moderate drought (60%~65% of
FWC) and the severe drought (35%~40% of FWC) groups were used. The Result showed that light response curves of the
TS and NS had no significant difference under these three soil moisture conditions. The fitting result showed good
performance by non-rectangular hyperbolic model. The net photosynthetic rate (Pmax), light saturation point (LSP) and
apparent quantum efficiency , dark respiration rate (Rd) of both TS and NS plants decreased significantly under drought
conditions and then continually increased. However, the decrease of TS plant was less than NS plant under stress condition
and the recovering was quicker. These results indicated that overexpressing antioxidant enzyme genes enhanced sweet
potato photosynthetic capacity, adaptability of drought stress and recovery capability. Light compensation point (LCP) of
sweet potato increased significantly under drought stress, but the rise in TS plants was less than NS control. These results
showed that transgenic genes to sweet potato enhanced its light environmental adaptability |
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