文章摘要
黄翠红,彭圣法,杨瑰丽,刘永柱,郭涛,王慧.厅项目(12zs2107)水稻籼型恢复系花药培养初步研究[J].广东农业科学,2014,41(3):13-16
查看全文    HTML 厅项目(12zs2107)水稻籼型恢复系花药培养初步研究
Research on photosynthetic characteristics of flagleaves in hybrid rice during grain filling
  
DOI:
中文关键词: 籼稻  恢复系  花药培养  基因型
英文关键词: hybrid rice  photosynthetic characteristic  leaf colour  grain yield
基金项目:国家863 计划项目(2012AA101201);现代农业 产业技术体系建设专项(CARS-01-12);广东省农业科技创新团 队项目(2011A020102004)
作者单位
黄翠红,彭圣法,杨瑰丽,刘永柱,郭涛,王慧 华南农业大学国家植物航天育种工程技术研究中心广东广州510642 
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中文摘要:
      通过对6个不同籼型恢复系水稻进行花药培养研究,先后分析了激素配比、有机附加物、不同基因型、 杂交组合等因素对花药培养力的影响。结果表明,B3 处理中激素配比(2,4-D颐NAA颐KT颐6-BA=2颐1颐0颐0)较为适用于 R8166 的花药培养,而B2 处理中激素配比(2,4-D颐NAA颐KT颐6-BA=2颐1颐0颐0.5)则较适用于航恢173;添加有机添加物能 在一定程度改善花药培养的愈伤诱导率,却降低其后续分化的能力。进一步研究表明,基因型是影响花药培养力的 最重要的内在因素,籼稻的花药培养力较低,其中泰丰占的花药培养力最高;杂交组合R1 和R2 的愈伤诱导率均比 R8166 有所提高,但后续分化则表现较差。通过试验逐步筛选出适合籼型恢复系水稻花药培养的优良培养条件,为 实现利用花药培养加快育种进程奠定基础。
英文摘要:
      The changes of chlorophyll content, photosynthetic parameters and chlorophyll fluorescence parameters of flag leaves in three hybrid rice combinations during grain filling were investigated. The results showed that Pn, Tr, Ci, Gs, Fv/Fm, ETR in leaves and Yield of different hybrid rice combinations during grain filling showed a wavelike decrease change with the development of seeds. The changes of these parameters were also significantly different. The Pn of Feiyou188 and B2You768 were much higher than Gangyou725 at the early stage of grain filling. Then, the Tr, Gs, Ci and Fv/Fm of Feiyou188 and B2You768 decreased gradually at the medium stage of grain filling, while these parameters of Gangyou725 were volatile during this period. The effective spike number, 1000-grain weight, grain yield of Feiyou188 and B2You768 were much higher than Gangyou725, showed better qualities.
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