| 丁唯嘉1，张穗强1，龚兵1，李春远1，王秀芳2.半红树植物苦槛蓝内生真菌的分离及
抗植物病原菌活性研究[J].广东农业科学,2014,41(3):74-78 |
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半红树植物苦槛蓝内生真菌的分离及
抗植物病原菌活性研究 |
| Quantitative characteristics of soil microorganism groupsin Hainan coastal Vatica mangachpoi forest |
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| DOI： |
| 中文关键词: 苦槛蓝 内生真菌 抗植物病原菌活性 |
| 英文关键词: Vatica mangachpoi physiological groups of bacteria quantitative distribution |
| 基金项目:家自然科学基金（21102049）；广东省自然科学基
金(9451064201003751，9151027501000055)；广东省高等学校高层
次人才项目（2011）；广州市科技计划项目（11C12100771）；华南农
业大学“211 工程”三期重点建设项目（2009B010100001）；国家留
学基金（201208440268） |
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| 摘要点击次数: 1805 |
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| 中文摘要: |
| 为寻找新的抗植物病原菌微生物资源，以苦槛蓝为对象开展了研究。从其叶、叶脉、茎、树皮、根共分离
到104 株内生真菌。经形态学初步鉴定，它们主要属于无孢类群、盘长孢属、青霉属、枝孢霉属和曲霉属。生物活性测
试表明，所分离到的苦槛蓝内生真菌中有77.9%对包括小麦赤霉、大豆疫霉、香蕉炭疽和柑橘青霉中至少1 种植物病
原菌显示抑制活性，42.3%和11.5%的菌株对上述植物病原菌中的至少1 种分别达到了中度抗菌和强烈抗菌。高效
液相测试显示苦槛蓝提取物与5 种具有强烈抗菌活性的苦槛蓝内生真菌提取物的谱图差异显著，表明这些内生真
菌产生了不同于原植物代谢产物的新的抗植物病原菌活性物质。 |
| 英文摘要: |
| In order to reveal the mechanism of natural regeneration in Shimei Bay coastal 灾atica mangachpoi forest
from the aspect of soil microorganism, the soil microorganisms in V. mangachpoi forest were investigated and analyzed.
Five-point sampling method was used to take the soil samples in both dry and wet seasons form rhizosphere and nonrhizosphere
soil, respectively, and the MPN was used to analyze the quantitative distribution of five physiological groups of
bacteria in the soil. The results showed that nitrite bacteria was not found in the soil of V. mangachpoi forest, there were
obvious differences among the quantities of other four physiological groups of bacteria, the quantities of ammonifying
bacteria >> aerobic nitrogens -fixing bacteria >> denitrifying bacteria >> aerobic cellulose decomposing bacteria; the
quantities of ammonifying bacteria, denitrifying bacteria and aerobic cellulose decomposing bacteria were less in the
rhizosphere soil than in the non-rhizosphere soil, but the quantity of aerobic nitrogens-fixing bacteria was opposite in the
rhizosphere and non-rhizosphere soil; the quantity of ammonifying bacteria was slightly higher in the subsoil than in the
upper soil, but quantities of the aerobic nitrogen-fixing bacteria, denitrifying bacteria and cellulose decomposing bacteria
were more concentrated in the upper soil. |
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