6种真菌诱导子对V3蝴蝶兰组培苗生长的影响

陈金花1; 石蕾2; 王存1; 杨光穗1

文章摘要

陈金花1，石蕾2，王存1，杨光穗1.6种真菌诱导子对V3蝴蝶兰组培苗生长的影响[J].广东农业科学,2014,41(4):48-51

查看全文 HTML 6种真菌诱导子对V3蝴蝶兰组培苗生长的影响

Effects of six kinds of fungal elicitor on plateletgrowth of Phalaenopsis V3 seedling

DOI：

中文关键词: 真菌诱导子蝴蝶兰组织培养

英文关键词: fungal elicitor曰Phalaenopsis曰tissue culture

基金项目:国家公益性行业（农业）科研专项（201203071）；海南省科技成果示范推广专项（SQ2013CGSF0006）；科技部农业科技成果转化项目（2011GB2E200006

作者	单位
陈金花1，石蕾2，王存1，杨光穗1	1、中国热带农业科学院热带作物品种资源研究所/农业部华南作物基因资源与种质创制重点实验室，海南儋州571737；2、南京林业大学森林资源与环境学院，江苏南京210037

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中文摘要:

为优化蝴蝶兰种苗快繁技术体系，探索一种无激素培养的蝴蝶兰组培方法，将从“生兰中分离得到的6 种菌根真菌制成真菌诱导子分别按40、60、80 mL/L 的剂量加入到基础培养基中，研究其对V3 蝴蝶兰组培苗生长的影响。结果表明院培养90 d后，处理1、2等13 个处理均能极显著地促进鲜重的增长，其中处理6、13、16 和处理18 效果最佳；处理9、10、13 的叶绿素a、叶绿素b、叶绿素(a+b)的含量均极显著高于CK。结合对鲜重和叶绿素的影响，添加40 mL/L 的Y5、80 mL/L 的Y3 最适合用于对蝴蝶兰组培苗的促生生产中。

英文摘要:

To optimize technical system of rapid propagation of Phalaenopsis seedling, we try to find a hormone-free method. This paper studied the effects of six kinds of fungal elicitor isolated from the wild Orchids爷s roots on platelet growth of Phalaenopsis V3 seedling. 40, 60, 80 ml/L fermentation liquid was added to the plant culture media, training 90 d, we analyzed fresh weight and chlorophyll content. Results showed that compared with the control group, 13 kinds of treat- ments was found to significantly increase the fresh weight (P<0.01), and that the inducing effects were in the order of trt6 > trt13>trt16>trt18. Compared with the control group, trt9, trt10 and trt13 were found to significantly increase the chlorophyll content (P<0.01). It can be concluded that, combined with the effect on fresh weight and chlorophyll, adding 40 ml/L Y5 or 80 ml/L Y3 is suited for the Phalaenopsis V3 seedling.

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