| 张志强，李永成.海巴戟天愈伤组织诱导及细胞悬浮培养试验[J].广东农业科学,2014,41(4):108-112 |
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海巴戟天愈伤组织诱导及细胞悬浮培养试验 |
| Study on callus induction and cell suspensionculture of Morinda citrifolia |
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| DOI： |
| 中文关键词: 海巴戟天 愈伤组织 悬浮培养 蒽醌 |
| 英文关键词: Morinda citrifolia callus suspension culture anthraquinone |
| 基金项目:海南省自然科学基金（311033 |
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| 摘要点击次数: 2038 |
| 全文下载次数: 619 |
| 中文摘要: |
| 以海巴戟天叶脉作为外植体，在B5 培养基上诱导出淡黄色、疏松愈伤组织，通过增殖后接种到液体培
养基中，建立细胞悬浮培养体系，并对影响细胞悬浮培养的培养基种类、接种量、pH 值、蔗糖含量等因素进行了研
究。结果表明院B5 + 1 mg/L 2,4-D + 0.1 mg/L 6-BA 能诱导出淡黄色、质地疏松的愈伤组织；较佳愈伤组织增殖培养
基为B5 + 2 mg/L NAA + 0.2 mg/L KT；细胞在B5 + 2 mg/L NAA + 0.2 mg/L KT 培养基中悬浮培养，接种量60 g/L（细
胞鲜重）、蔗糖4%、pH 值5.8、100 r/min、27益下暗培养，海巴戟天悬浮细胞比生长速率为0.0836 d-1，总蒽醌含量
367.8 mg/L，培养周期27~30 d。 |
| 英文摘要: |
| Faint yellow and loose callus was induced from vein of Morinda citrifolia on B5 medium. After proliferation,
the callus was inoculated in liquid medium and cell suspension lines were established. Medium variety, inoculation
densities, pH and sucrose concentration were optimized in this research. The results indicated that, faint yellow and loose
callus could be induced on B5 medium by adding 1 mg/L 2,4-D and 0.1 mg/L 6-BA; B5 medium by adding 2 mg/L NAA
and 0.2 mg/L KT was the better callus proliferation medium. Cells could grow well with 2 mg/L NAA and 0.2 mg/L KT in
B5 medium with 60 g/L (fresh cell weight) of inoculation densities, 4% sucrose, pH 5.8, 100 r/min, and 27~28益in dark.
The specific growth rate was 0.0836 d-1, and anthraquinone production could reach 367.8 mg/L after 27~30 d of suspension
culture. |
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