| 张森，刘平怀，王影，罗宁，张玲，杨勋.铁、镁及钙对微藻Desmodesmus sp. WC08
生长和油脂积累的影响[J].广东农业科学,2014,41(4):126-130 |
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铁、镁及钙对微藻Desmodesmus sp. WC08
生长和油脂积累的影响 |
| Effects of concentrations of iron, calcium and magnesiumon the growth and lipid accumulation of microalgalstrain Desmodesmus sp. WC08 |
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| DOI： |
| 中文关键词: Desmodesmus sp. 铁 镁 钙 生长 油 |
| 英文关键词: Desmodesmus sp iron calcium magnesium growth lipid |
| 基金项目:国家科技支撑计划项目（2011BAD14B01）；海南
省中药现代化科技专项（ZY201327）；海南大学植物学国家级重
点学科（071001） |
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| 中文摘要: |
| 采用单因素试验考察微藻Desmodesmus sp. WC08 在4 种培养基中的生长和油脂积累的，并以海水
BG11 为基础培养基，研究铁、镁及钙浓度对该海水微藻生长和油脂积累的影响。结果表明，Desmodesmus sp. WC08
在海水BG11 中的生物量和油脂产量最高，分别为2.42 g/L 和0.58 g/L，在F/2 中的油脂含量最高，为40.80%。铁、镁
及钙浓度试验表明，钙浓度对Desmodesmus sp. WC08 生物量具有显著影响，而铁浓度和镁浓度的影响较小。同时，铁
浓度和钙浓度对其油脂含量具有显著影响，而镁浓度对其油脂含量影响较小。综合考虑，铁、镁及钙对其油脂产量均
具有显著影响。Desmodesmus sp. WC08 最适生长和油脂积累的培养基为海水BG11，且其铁、镁及钙浓度分别为2伊
10-5，0.09 及0.05 mmol/L 时，最利于Desmodesmus sp. WC08 的生长和油脂积累。 |
| 英文摘要: |
| To investigate the effects of concentration of iron, calcium and magnesium on the growth and lipid
accumulation of microalgal strain Desmodesmus sp. WC08, this research has tested the effects of 4 culture medium on the
growth and lipid accumulation by single factor experiment. It implied that the optimal biomass (2.42 g/L) and lipid productive
(0.58 g/L) were obtained in brine BG11, but the highest oil content (40.80%) was gained in F/2. Iron, magnesium and calcium
concentrations tests showed that calcium concentration had the significant effect on the biomass of Desmodesmus sp. WC08,
and minor effect was for iron concentration and magnesium concentration. At the same time, iron and calcium concentration
had significant effects on the oil content, but magnesium concentration just had smaller influence on the oil content. Given
lipid productive, the iron, magnesium and calcium all had significant impact. Brine BG11 medium was the optimal culture
medium for the growth and oil accumulation of Desmodesmus sp. WC08. And the optimal iron, magnesium and calcium
concentrations in brine BG11 culture medium were 2伊10-5, 0.09 and 0.05 mmol/L, respectively. |
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