| 高萍，钟玉宜，张爱玲.母猪卵巢颗粒细胞的分离培养及鉴定[J].广东农业科学,2014,41(4):131-135 |
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母猪卵巢颗粒细胞的分离培养及鉴定 |
| Separation, culture and identificationof sow ovarian granulose cells |
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| DOI： |
| 中文关键词: 猪卵巢颗粒细胞 细胞培养 免疫荧光 细胞周期 细胞活力 |
| 英文关键词: sow ovarian granulose cells cell culture immune-fluorescence cell cycle cell vitality |
| 基金项目:国家自然科学基金（31201771） |
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| 摘要点击次数: 1875 |
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| 中文摘要: |
| 颗粒细胞是卵巢卵泡重要的结构组分，其形态、功能伴随原始卵泡的生长启动、增殖、分化、闭锁、排卵
以及黄体形成等生理过程发生各种变化。颗粒细胞是研究细胞增殖、分化和细胞相互作用、信号转导通路的理想细
胞模型。利用注射器吸取有腔卵泡卵泡液，成功分离培养了猪有腔卵泡的颗粒细胞，并进行了FSHR 免疫荧光检测
以及原代猪卵巢颗粒细胞生长活力检测，结果表明院该细胞对FSHR 的免疫荧光呈阳性。流式细胞仪检测发现该细
胞的细胞周期为24 h，但在培养4 d时细胞的活力最强。该研究结果为进一步研究母猪卵巢卵泡功能奠定了基础。 |
| 英文摘要: |
| Granulose cells aer important structural constituent of mammalian ovary, their morphology and function change
following the physiological processes of primordial follicular activation, cell proliferation and differentiation, follicular atresia,
ovulation and luteinization. At the same time, granulose cell is the usual model to analyze cell interaction and signal transduction
of the follicular. Sterile syringes were used to absorb the fluid from the antral follicle. The cells separated and cultured from the
follicular fluid were devoted to the FSHR immune-fluorescence identification, cell cycle detection and vitality test. The results
demonstrated that cells separated from the antral follicle fluid were granules cells, were positive in the FSHR anti -body.
Furthermore, the cell cycle of granulose cell was 24 h, and the most highly cell vitality recched at the 4th day. |
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