| 程保平1，赵弘巍2，彭埃天1，陈霞1，宋晓兵1，凌金锋1.以omp基因为靶标的3 种检测方法对
柑橘黄龙病菌检测效果比较[J].广东农业科学,2014,41(5):141-145 |
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以omp基因为靶标的3 种检测方法对
柑橘黄龙病菌检测效果比较 |
| Comparative study on citrus huanglongbing moleculardetection methods with the omp target gene |
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| DOI： |
| 中文关键词: 柑橘黄龙病 常规PCR LAMP 实时荧光定量PCR |
| 英文关键词: citrus huanglongbing conventional PCR LAMP realtime quantitative PCR |
| 基金项目:广东省农业科技推广重大技术研究项目（2012011
27）；广东省现代农业产业技术体系建设专项(粤农[2009]380 号)；
广东省农业科学院院长基金（201305）；广东省科技创新人才出国
培养计划项目（粤农科[2013]74 号） |
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| 中文摘要: |
| 为了比较以omp 基因为靶标的常规PCR、LAMP 检测方法（Loop-mediated isothermal amplification）和实
时荧光定量PCR 方法在实际检测中对柑橘黄龙病的检测效果，比较了3 种检测方法对柑橘黄龙病菌omp 基因检测
的灵敏度，并运用3 种检测方法对采自广东省田间11 个本地柑橘品种上疑似感染黄龙病的多个病样进行实际检
测。结果显示院3 种检测方法的灵敏度有所不同，依次为常规PCR |
| 英文摘要: |
| Detection sensitivity and detection rate of conventional PCR, LAMP and real-time quantitative PCR were
compared with the omp target gene. The results showed that LAMP had similar detection sensitivity with real -time
quantitative PCR. And both of the two detection methods had higher detection sensitivity than that of the conventional
PCR. Then total 35 suspected citrus samples from different cultivers with symptom of HLB in orchard of Guangdong were
amplified and confirmed by conventional PCR, LAMP and real-time quantitative PCR. The results showed that LAMP had
similar detection rate with real-time quantitative PCR. And both of the two detection methods had higher detection rate
than that of the conventional PCR. This research indicated that conventional PCR could be used in large scale diagnosis of
HLB disease, real-time quantitative PCR had the highest detection sensitivity, and LAMP was simple, fast and didn爷t rely
on the expensive equipments, had good application prospect. |
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