| 徐匆1，李艳芳1，黄皓1，梁卫驱1，胡珊1，陈仕丽1，罗鸿斌2，罗华建1.酶定向进化文库的筛选方法[J].广东农业科学,2014,41(5):194-197 |
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酶定向进化文库的筛选方法 |
| Screening method of the directed evolution library of enzyme |
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| DOI： |
| 中文关键词: 酶定向进化 筛选方法 FASC |
| 英文关键词: directed evolution of enzyme screening method FASC |
| 基金项目:东莞市高等院校科研机构研发项目（2009108101
12）；东莞市科技计划项目(2007108101108) |
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| 中文摘要: |
| 酶工程作为生物技术的主体之一，对农产品加工等的发展具有广泛作用。自然界中的酶往往不能满足
实际生产，因此需要通过模拟自然界中的蛋白质进化，对酶进行改造和突变文库筛选，最终得到符合实际生产的酶。
目前，改造酶的途径主要有定点突变和定向突变两种。突变体文库的建立方法较为成熟，并且建立起的文库库容也
能够达到研究者的要求，但是突变体文库的筛选受现有技术等所局限，成为了定向进化技术的瓶颈。介绍了几种常
用的突变体文库的筛选方法，对它们的原理及特点进行阐述，并对未来的发展方向及趋势进行了总结。 |
| 英文摘要: |
| Enzyme engineering is one of the subject in biological technology, has a widely effect in agricultural
products processing. Natural enzymes often cannot fit the actual production, so the enzyme modification and mutant library
screening are necessary. At present, there are two main ways for enzyme evolution, including point mutation and directed
mutation. Establishment methods of mutant libraries are mature relatively, and the capacity of libraries can meet the
requirements. But the screening methods of mutant libraries are restricted by existing techniques, and become the
bottleneck of the directed evolution technique. This paper introduces several common methods for mutant library screening,
elaborates the principle and characteristics of these methods, and the future development direction and trend are
summarized. |
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