| 吕平1，张继1，檀小辉1，程琴1，庞新华1，2袁金刚1，苏文潘1，黄惠芳1.甘蔗03-75 品系脱毒苗培育技术研究[J].广东农业科学,2014,41(6):32-34 |
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甘蔗03-75 品系脱毒苗培育技术研究 |
| Study on cultivation techniques for virus-freeseedling of sugarcane 03-75 strains |
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| DOI： |
| 中文关键词: 甘蔗 组织培养 热处理 脱毒 |
| 英文关键词: sugarcane tissue culture hot water treatment virus-free |
| 基金项目:国家十二五科技支撑计划项目2013BAC2B03 |
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| 摘要点击次数: 2064 |
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| 中文摘要: |
| 为探讨甘蔗脱毒苗的培育技术，以甘蔗03-75 品系为试验材料，经过52（依0.2）益水浴热处理及培养后，
对甘蔗心叶的诱导培养。愈伤组织分化、生根培养基的筛选，以及脱毒苗炼苗尧移栽及病毒检测方法等进行研究。结
果表明：采用热处理结合心叶脱毒的方法能有效去除甘蔗病害，不同培养阶段对激素种类、质量浓度有差异，其中
MS+2,4-D 3.0 mg/L+NAA 0.1 mg/L 培养基对愈伤组织诱导、继代较适宜，MS+ BA 1.0 mg/L + NAA 0.1 mg/L 培养基对
愈伤组织分化效果好，1/2MS 改良+NAA 4.0 mg/L +MET 1.0 mg/L 培养基生根率高、根系粗壮；组培苗通过利用分子
生物学技术（PCR）对甘蔗花叶病及酶联免疫吸附法( ELISA)对甘蔗宿根矮化病进行检测袁结果呈阴性。 |
| 英文摘要: |
| In order to study the cultivation techniques for virus-free seedling of sugarcane, taking sugarcane 03-75
strains as materials, after the water bath of 52(依0.2)益and culturing, the induction culture of sugarcane leaves, callus
differentiation, screening of rooting medium, and the methods of acclimatization, transplanting and virus detection for virusfree
seedlings were discussed. The results showed that the method of heat treatment combined with seedling leaves
detoxication could remove sugarcane Mosaic, perennial root dwarf disease and other diseases effectively. The hormone types
and concentrations were different in different stages, the best medium for callus induction and subculture was MS+2,4-D
3.0 mg/L+NAA 0.1 mg/L, for callus differentiation was MS+ BA 1.0 mg/L + NAA 0.1 mg/L, and 1/2MS+NAA 4.0 mg/L +
MET 1.0 mg/L was good for high rooting rate and root stout. By using molecular biology techniques (PCR) for sugarcane
Mosaic disease detection and enzyme linked immunosorbent (ELISA) for sugarcane perennial root dwarfing detection of
tissue culture plants, the results were negative |
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