| 李丽丽1，杨洪一2.苹果茎痘病毒种群遗传多样性分析[J].广东农业科学,2014,41(6):93-95 |
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苹果茎痘病毒种群遗传多样性分析 |
| Genetic polymorphism analysis of Applestem pitting virus population |
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| DOI： |
| 中文关键词: 苹果茎痘病毒 种群 核苷酸多样性 |
| 英文关键词: Apple stem pitting virus population nucleotide diversities |
| 基金项目:中央高校基本科研业务费(DL13EA06-3) |
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| 中文摘要: |
| 苹果茎痘病毒(Apple stem pitting virus, ASPV)是一种严重危害果树生产的潜隐性病毒。基于前期研究所获得的6 个ASPV 中国分离物CP基因的核苷酸序列，结合GenBank 中报道的序列，对ASPV 种群遗传多样性特点进行分析。应用MEGA 4.0 软件分析了不同地理来源、不同寄主来源组内和组间的各分离物CP基因的遗传多样性，表明ASPV 种群遗传变异与寄主来源、地理分布不存在明显的相关性。CP编码区仍处于净化选择中，亚洲的分离物、梨分离物承受着较高的选择压力。DnaSP 软件分析显示，ASPV 经历了明显的种群扩张，并有继续扩展蔓延之势。因而有必要进一步加强病毒检测及种苗脱毒，严格控制带毒种苗传播。 |
| 英文摘要: |
| Apple stem pitting virus (ASPV) is one of the most important latent viruses that infects fruit trees. Based on
the nucleotide sequence of coat protein (CP) gene from 6 Chinese isolates and other isolates from GenBank, the
characteristics of genetic polymorphism of ASPV population were analyzed. The within-population and between-population
nucleotide diversities of CP gene among different geographical and hosts origins were analyzed with MEGA 4.0. The results
indicated that there were not various relativities between the genetic polymorphism and the geographical and hosts origins.
The variation of CP gene was located in purifying selection, and the Asian isolates and pear isolates of ASPV had higher
pressure of selection. The analysis of DnaSP software showed that ASPV suffered various population dilatation and the
dilatation would continue to develop. Therefore, it need further enhance the detection of virus and elimination of virus for
seedlings and strictly control the spread of seedlings infected by ASPV. Key words: Apple stem pitting virus; population; nucleotide diversities |
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