| 刘南南1，王宝琴1，管宇2.根癌农杆菌介导反义F3'H基因
对矮牵牛的遗传转化[J].广东农业科学,2014,41(6):150-153 |
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根癌农杆菌介导反义F3'H基因
对矮牵牛的遗传转化 |
| Agrobacterium-mediated genetic transformation ofantisense F3'H gene into Petunia hybrida Vilm |
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| DOI： |
| 中文关键词: 矮牵牛 F3'H 基因 根癌农杆菌 遗传转化 |
| 英文关键词: Petunia hybrida Vilm F3'H gene Agrobacterium tumefaciens genetic transformation |
| 基金项目:山东省自然科学基金（Y2008D39）；滨州学院博
士科研启动项目（2007Y10） |
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| 摘要点击次数: 1710 |
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| 中文摘要: |
| 以矮牵牛无菌苗叶片作为转化受体材料，建立并优化了农杆菌介导的矮牵牛遗传转化体系。通过农杆菌介导法将水稻反义类黄酮3'-羟化酶基因（F3'H）转入矮牵牛中，抗性植株经PCR 检测均为阳性植株，初步表明反义F3'H 基因已整合到矮牵牛基因组中；对阳性植株进行半定量RT-PCR 检测，与非转基因植株相比，阳性植株中F3'H 基因的表达减弱，表明导入的反义F3'H 基因抑制了内源基因的表达。 |
| 英文摘要: |
| Agrobacterium-mediated transformation system of Petunia hybrida was estabilished and optimized with P.
hybrida leaf as the material. Antisense F3'H gene was transfored into Petunia by Agrobacterium-mediated transformation
system. The resistant plants were all positive plants by PCR detection, which indicated antisense F3'H gene had been
integrated into Petunia genome. Compared with non transgenic plants, F3'H gene expression in the positive plants detected
by semi-quantitative RT-PCR was abate, which showed that the import of antisense F3'H gene inhibited the expression of
endogenous genes. |
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