| 乔培培1,2，陈丕茂1，冯雪1.高盐度海水氮磷营养盐保存技术研究[J].广东农业科学,2014,41(7):114-119 |
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高盐度海水氮磷营养盐保存技术研究 |
| Study on preservation method of nitrogen andphosphorous in high salinity seawater |
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| DOI： |
| 中文关键词: 海水 盐度 氮磷营养盐 保存方法 |
| 英文关键词: seawater salinity nitrogen and phosphorus nutrient preservation method |
| 基金项目:国家科技支撑计划项目(2012BAD18B02）；国家
公益性行业(农业）科研专项(201003068）；中国水产科学研究：
：级基本科研业务费专项(2012A0102） |
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| 摘要点击次数: 1787 |
| 全文下载次数: 654 |
| 中文摘要: |
| 采用12 种不同方法保存盐度为32 的高盐度海水水样中的4 种氮磷营养盐(NH3-N、NO2-N、NO3-N、
PO4-P），使用FIAstar5000 营养盐流动注射分析仪对4 种营养盐在9 d保存期内参数值的稳定性进行研究。结果表
明，不同保存方法间存在差异，每种营养盐的最佳保存条件不同。9 d 试验期内，水样中NH3-N 的变化趋势较大，
NH3-N 和PO4-P 的含量呈减小趋势，NO2-N、NO3-N 的含量略微上升。水样中加氯仿对氮营养盐(NH3-N、NO2-N、
NO3-N）的保存效果较好，加甲醛对磷营养盐(PO4-P）的保存效果较好。综合考虑试验结果的有效性和可操作性，得出
了4 种营养盐9 d内最适保存条件：常温下加5译氯仿，NH3-N 的保存效果能稳定在80%以上；在-20益加1译氯仿
时，NO3-N 的保存效果最好，9 d内稳定性都在95%以上；在-20益或4益下加5译或1译氯仿，NO2-N 在9 d内都能稳
定在95%以上；4益下加5译甲醛时，PO4-P 的保存效果最好。 |
| 英文摘要: |
| In this paper, 12 different preservation methods were used to save 4 kinds of nitrogen and phosphorus
nutrients (NH3-N, NO2-N, NO3-N, PO4-P) in high salinity sea water of salinity 32. We studied the parameter values'
stability of 4 kinds of nutrients in 9 days storage using FIAstar5000 nutrient flow injection analyzer. The results showed
that there were obvious differences among different preservation methods, the best preservation conditions of each nutrient
was different. In 9 days experimental period, NH3-N in water samples had a large change tend, the NH4-N and PO4-P
content presented a trend of decrease, while NO2-N and NO3-N content slightly rose. When chloroform was added into
water samples, it was very good for the preservation of nitrogen nutrient (NH3-N, NO2-N and NO3-N); when formaldehyde
was added, it was good for the preservation of phosphorus nutrient (PO4-P). Considered the effectiveness and operability of
the experiment results, we obtained the optimal preservation condition of the 4 kinds of nutrient in 9 days. When 5译
chloroform was added into water samples, the stability of NH3-N could be controlled more than 80%; when 1 chloroform
was added into water samples and the water samples were stored at -20, the stability of NO3-N could be over 95%; when
5译chloroform was added at -20 or 1译chloroform was added at 4益into water samples, the NO2-N could be more than
95%; when 5译formaldehyde was added at 4, it was the best method to save PO4-P. |
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