| 唐志敏，黄士华，江冬，古远安，杨礼香.mASR3基因超量表达载体的构建[J].广东农业科学,2014,41(7):137-141 |
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mASR3基因超量表达载体的构建 |
| Over-expression vector construction of mASR3 gene |
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| DOI： |
| 中文关键词: mASR3 超量表达载体 转基因植株 |
| 英文关键词: mASR3 over-expression vector transgenic plant |
| 基金项目:国家级大学生创新训练项目(201211078019） |
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| 摘要点击次数: 1292 |
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| 中文摘要: |
| ASR 是植物特异的亲水蛋白家族，近来发现它们在植物生长发育、成熟衰老以及非生物胁迫过程中起着
重要的作用。而mASR3 基因是从植物中发现的一种转录因子，为进一步研究该基因功能，将合成的mASR3 基因构
建入超表达载体pMD1 中，并通过农杆菌将此重组质粒转化入拟南芥，转入目的基因的拟南芥子叶为绿色，下胚轴
较长，并具有较长的主根，筛选得到的转基因植株，为该基因的进一步功能研究打下基础。 |
| 英文摘要: |
| ASR is a specific hydrophilic protein family in plant, playing important role in plant growth and
development, ripening and senescence and abiotic stress. And mASR3 gene is a kind of transcription factors found in plant
in recent years. In order to further study its function, the synthesized gene mASR3 was cloned into over-expression vector
(pMD1), formed a recombinant plasmid called pMD1-mASR3. The recombinant plasmid was transformed into Arabidopsis
thaliana through Agrobacterium tumefaciens. The cotyledon was green, hypocotyls was longer and the taproot was longer in
the transformed Arabidopsis. The transgenic plants laid the foundation for its further study of function. |
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