| 陈善真1，潘忠超1，段元慧2，王志花1，刘春红2，石和荣1，李其昌1，王贵平1.一株尼罗罗非鱼致病性嗜水气单胞菌的
分离鉴定及药敏试验[J].广东农业科学,2014,41(7):158-161 |
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一株尼罗罗非鱼致病性嗜水气单胞菌的
分离鉴定及药敏试验 |
| Isolation, identification and drug sensitivity test of pathogenicAeromonas hydrophila from Oreochromis niloticus |
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| DOI： |
| 中文关键词: 嗜水气单胞菌 分离鉴定 药敏试验 致病性 |
| 英文关键词: Aeromonas hydrophila isolation and identification drug sensitivity test pathogenicity |
| 基金项目:广东省海洋渔业科技推广专项(粤财农[2011]587
号）；番禺区科技计划项目(2011-Z-02-07） |
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| 摘要点击次数: 1454 |
| 全文下载次数: 586 |
| 中文摘要: |
| 从自然感染发病的尼罗罗非鱼肝脏和脑组织中分离出1 株细菌，经革兰氏染色镜检、生化鉴定和PCR
鉴定，确定为嗜水气单胞菌，命名为GD001，进一步采用药敏纸片法测定分离菌株对多种抗生素的敏感性，并用该菌
株接种健康尼罗罗非鱼，鉴定其致病力。结果发现，GD001 菌株对恩诺沙星、头孢噻吩、四环素和克莱霉素等抗生素
敏感，对链霉素轻度敏感，对卡那霉素和青霉素不敏感。从人工感染10 g 左右的健康罗非鱼发现，GD001 菌株具有很
强的致病力，试验鱼在感染后3~4 h内全部死亡，且患病症状与自然发病症状的鱼一致。 |
| 英文摘要: |
| In this study, we isolated a bacterial strain named GD001 from liver and brain of natural infection
Oreochromis niloticus. By gram staining, biochemical test and PCR identification, it was confirmed Aeromonas hydrophila.
The results of drug sensitivity test indicated that the isolated strain was sensitive to enrofloxacin, cephalothin, tetracycline,
clarith, light sensitive to streptomycin, and resistant to kanamycin and penicillin. The GD001 was used to infect healthy O.
niloticus about 10 g, all infected fish died in 3-4 hours and the symptoms of the infected fish were consistent with the
natural diseased fish. |
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