| 高建明1，胡彩平1，郑金龙2，张世清1，陈河龙1，刘巧莲1，习金根2，易克贤1，2.柱花草炭疽菌突变体库致病缺陷转化子筛选
及T-DNA插入位点侧翼序列分析[J].广东农业科学,2014,41(8):189-191 |
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柱花草炭疽菌突变体库致病缺陷转化子筛选
及T-DNA插入位点侧翼序列分析 |
| Screening of pathogenicity defect mutant in Colletotrichumgloeosporioides of Stylosanthes guianensias and molecularcloning of T-DNA integration flanking sequence |
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| DOI： |
| 中文关键词: 炭疽菌 突变体 致病缺陷 筛选 侧翼序列 |
| 英文关键词: Colletotrichum gloeosporioides mutant pathogenicity defect screening flanking sequence |
| 基金项目:国家自然科学基金(31072076） |
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| 摘要点击次数: 1691 |
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| 中文摘要: |
| 比较了接种病原菌侵染柱花草的几种方法的效果，选定出最佳的初筛和复筛方法，进而对1 230 个转化
子进行筛选，得到致病力缺陷菌23 株，其中致病力减弱的菌株18 株，致病力完全丧失的5 株。利用TAIL-PCR 扩增
致病力丧失突变子t-2430 的T-DNA 插入位点侧翼序列，得到大小为476 bp 的一段序列，BLAST 比对已测序炭疽菌
基因组，发现401nt 和数据库的Contig464的部分序列完全一致，进一步对该段序列的功能进行预测，表明T-DNA 插入
在一预测基因的启动子区域，并且这个预测基因与稻瘟病菌(Magnaporthe oryzae）的天冬氨酸转氨酶(XP_003719674.1）同
源性为79%。 |
| 英文摘要: |
| Several inoculation methods were compared to test their effect of pathogenicity to Stylosanthes guianensias.
In all 1 230 mutants, 23 had decreased weak pathogenicity, 5 even completely losted its ability to infect S. guianensias.
TAIL-PCR was used to identify the T-DNA integration site in the mutant that was coded t-2430 and the sequence of 476
bp was gotten. The flanking sequence of T-DNA was compared with Colletotrichum genome by blast and 401nt of sequence
was found in Contig464 of Colletotrichum genome. By predicting the function of flanking sequence, we found T-DNA
insertion in the promoter region of putative gene, which had 79% homology to Magnaporthe oryzae aspartate
aminotransferase gene (XP_003719674.1). |
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