杨锐培1,许善雄1,陈蔚1,赖小平1,何国振1,2.海南砂生殖生物学特性研究[J].广东农业科学,2014,41(9):20- |
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海南砂生殖生物学特性研究 |
Study on reproductive biology characteristicsof Amomum longiligulare T Wu |
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DOI: |
中文关键词: 海南砂 花粉活力 柱头可授性 生殖生物学特性 |
英文关键词: Amomum longiligulare pollen viability stigma receptivity reproductive biology characteristics |
基金项目:国家科技支撑计划项目(2011BAI01B02);海南省
中药现代化专项(2011ZY003);海南省星火产业带专项(H
NXH201321) |
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中文摘要: |
为了提高海南砂的产量,研究了其小花数目、花粉活力、柱头可授性、落果率等生殖生物学特性以及海
南砂花粉在不同贮藏条件下的活力。结果表明,海南砂每个花序有7.28(依0.83)朵小花;小花的花期为1.5 d;离体萌
发法测定花粉活力在花朵刚刚开放时达83.6%,开花12 h后降为21.0%,24 h后降为11.4%;柱头和花粉在花朵开放
6 h 内都具极强可授性,寿命维持24 h,比花粉寿命长12 h以上;人工授粉率和自然授粉率分别为64.10(依29.80)%
和17.55(依15.49)%、落果率为20.65(依31.39)%和11.11(依32.34)%。高湿度条件下贮藏花粉12 h后活力降为0.6%,而
在低温高湿条件下贮藏花粉12 h的活力为23.4%,表明低温更有利于海南砂花粉贮藏。 |
英文摘要: |
In order to improve the yield of Amomum longiligulare, some characteristics of reproductive biology,
including floweret number, pollen viability, stigma receptivity and fruit-dropping rate, were studied. The storage methods
for pollens under different conditions were investigated as well. The results showed that the floweret number of A .
longiligulare was 7.28 (0.83) in a anthotaxy. The floweret blossom was 1.5 day. The pollen viabilities in vitro were 83.6%,
21.0% and 11.4% at flowering time of 0 h, 12 h and 24 h detected by germination method. The stigma receptivity was
strong within 6 h after flowering, and lasted 24 h, which was more longer than the lifespan of the pollen at least 12 h.
Artificial pollinating rate and natural pollinating rate were 64.10(29.80)% and 17.55(15.49)%, respectively. In the cases
of artificial and natural pollination, the fruit dropping rates were 20.65(31.39)% and 11.11(32.34)%. When pollens were
stored for 12 h, the pollen viability decreased to 0.6% under the condition of high humidity, whereas it was still 23.4%
under the condition of cold and high humidity. This result indicates that the lifespan of A. longiligulare pollen can be
prolonged significantly in the low temperature environment. |
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