| 葛国平，吴亮宇，张少雄，林金科.茶树芽叶儿茶素含量与转录因子
MYB、bHLH关联分析[J].广东农业科学,2014,41(9):28- |
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茶树芽叶儿茶素含量与转录因子
MYB、bHLH关联分析 |
| Correlation analysis between catechins content and transcriptionfactor MYB, bHLH of bud and leaf in tea plant |
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| DOI： |
| 中文关键词: 茶树 儿茶素 MYB bHLH 关联性 |
| 英文关键词: tea plant catechins MYB bHLH correlation |
| 基金项目:国家自然科学基金（31170651) |
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| 摘要点击次数: 1503 |
| 全文下载次数: 700 |
| 中文摘要: |
| 以茶树芽叶为试验材料，用高效液相色谱检测儿茶素含量，用高通量测序技术分析转录因子MYB、
bHLH 表达差异，应用数据统计分析儿茶素含量与转录因子MYB、bHLH 表达的关联性。结果表明院3 个转录因子
MYB 基因comp150334_c1、comp143073_c0、comp130756_c0 与儿茶素总量、酯型儿茶素含量的关联系数均在0.80 以
上；3 个转录因子MYB 基因comp166178_c0、comp159173_c0、comp109329_c0，3 个转录因子bHLH 基因
comp159534_c2、comp159225_c0、comp142932_c0 与儿茶素总量、酯型儿茶素含量的关联系数均在0.75 以上，这将为
研究茶树儿茶素生物合成过程的关键转录因子基因提供理论依据。 |
| 英文摘要: |
| Using buds and leaves from tea plant as experimental materials, their catechins contents were detected by
HPLC, differential expression of transcription factor MYB, bHLH were analyzed by high-throughput sequencing, and then
the correlation between catechins content and transcription factor MYB, bHLH expression were analyzed. The results
indicated that the correlation coefficient of total catechin contents, ester catechin contents with three MYB transcription
factor genes comp150334_c1, comp143073_c0, comp130756_c0 were above 0.80; The correlation coefficient of total
catechin contents, ester catechin contents with three MYB transcription factor genes comp166178_c0, comp159173_c0,
comp109329_c0, three bHLH transcription factor genes comp159534_c2, comp159225_c0, comp142932_c0 were above
0.75. The results provide theoretical basis for the key transcription factor genes of catechins biosynthesis in tea plant. |
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