| 吴荣，樊国盛，王锦，林萍.铁线莲愈伤组织及不定芽诱导试验[J].广东农业科学,2014,41(9):48- |
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铁线莲愈伤组织及不定芽诱导试验 |
| Study on induction of callus and adventitionsbuds in Clematis L. |
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| DOI： |
| 中文关键词: 诱导 愈伤组织 不定芽 铁线莲Gipsy Queen |
| 英文关键词: induction callus adventitions bud Clematis Gipsy Queen |
| 基金项目:国家林业局“948”项目（2008-4-11）；云南省教
育厅科研基金（09Y0308）；西南林业大学面上科研基金（2009M
28）；云南省高校园林植物与观赏园艺重点实验室项目 |
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| 摘要点击次数: 1552 |
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| 中文摘要: |
| 以铁线莲Gipsy Queen的茎节和茎段为外植体，在MS基本培养基中添加0、1.0、2.0 mg/L 6-BA 和0、0.01、
0.05 mg/L NAA进行初代诱导和继代增殖培养。结果表明院最适合诱导愈伤组织的培养基为MS+NAA 0.01 mg/L+6-BA
2.0 mg/L，诱导率可达50.0%，诱导出的愈伤组织呈淡黄色疏松颗粒状；最适合诱导侧芽的培养基为MS+NAA 0.01
mg/L+6-BA 1.0 mg/L，出芽率可达170%；最适增殖培养基为MS+NAA 0.01 mg/L+6-BA 2.0 mg/L，增殖倍数达3.39。 |
| 英文摘要: |
| Callus and adventitions bud of Gipsy Queen were induced with columella and stem of Gipsy Queen as
explants and with the MS added different concentrations of 6-BA (0, 1.0, 2.0 mg/L) and NAA (0, 0.01, 0.05 mg/L) as
nutrient medium. The results showed that the callus could be easily induced in MS+NAA 0.01 mg/L+6-BA 2.0 mg/L, the
induction rate was 50.0%, and the induced callus was loose and granular appeared yellowish. The best lateral bud induced
medium was MS+NAA 0.01 mg/L+6-BA 1.0 mg/L, and the budding rate was 170%. The best multiplied medium was MS+
NAA 0.01 mg/L+6-BA 2.0 mg/L, and the proliferation times was up to 3.39. |
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