| 赵志祥，陈圆，肖敏，肖彤斌，陈绵才.海南淮山茎腐病病原生物学特性研究[J].广东农业科学,2014,41(9):95- |
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海南淮山茎腐病病原生物学特性研究 |
| Study on biological characteristics of dioscoreastem rot disease in Hainan |
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| DOI： |
| 中文关键词: 淮山茎腐病菌 菌丝生长 孢子萌发 |
| 英文关键词: Fusarium oxysporum f. sp. dioscorea mycelium growth spore germination |
| 基金项目:海南省科学事业费项目（12-214001-0004）；“十
二五”国家科技支撑计划项目（2012BAD19B06 |
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| 中文摘要: |
| 淮山茎腐病是近年来发生在海南淮山中的一种重要病害，对海南海口、临高等地的淮山生产造成严重
危害。通过菌丝生长速率法测定了培养基、温度、酸碱度、光照对淮山茎腐病菌菌丝生长的影响，同时，测定了湿度和
养分对孢子萌发的影响。结果表明院PDA 培养基最适合菌丝的生长；菌丝能够生长的温度范围为10~35益，最适温度
为25益，致死条件为50益处理10 min 或55益处理5 min；最适pH 值为7.0，菌丝能够生长的pH 值范围为5~10；光照
条件则以黑暗条件下最佳，而孢子萌发的最佳相对湿度为100%，溶液成分对孢子萌发的影响不大。 |
| 英文摘要: |
| Dioscorea stem rot disease is a serious disease infected dioscorea in Hainan recently. At present, it has
been broken out in Haikou and Lingao of Hainan, and caused serious economic losses to the dioscorea production in
Hainan. In order to understand its biological characteristics, and to provide references for prevention and control of this
disease, the method of mycelium growth rate was used to determine the influence of medium, temperature, pH value and
illumination on mycelium growth. Also, the influence of moisture and nutrient on spore germination was assayed. The
results showed that the most suitable culture medium was PDA and optimum temperature was 25. The temperature of
the mycelium growth ranged from 10 to 35. And lethal temperature was 50益for 10 min or 55益for 5 min. The
optimal pH value was 7.0. The pH value of mycelium growth ranged from 5 to 10. Dim light conditions was the best
lighting condition, and the best relative humidity was 100% for spore germination, solution composition had little effect
on spore germination. |
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