| 尹群健，陈潇骁，杨宏胜，林明师，毛欣琪，方再光.琼胶降解菌产酶条件优化、酶学性质和糖代谢途径[J].广东农业科学,2014,41(9):117- |
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琼胶降解菌产酶条件优化、酶学性质和糖代谢途径 |
| Optimization of fermentation conditions, enzymatic propertiesand sugar metabolic pathways of agarase-producing strain |
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| DOI： |
| 中文关键词: 琼胶降解菌 琼胶酶 产酶条件 酶学性质 糖代谢途径 |
| 英文关键词: agarase-producing strain agarase fermentation conditions enzymatic properties sugar metabolic pathways |
| 基金项目:国家自然科学基金（40666001）；海南大学青年基
金（qnjj1205）；校县合作项目（02005001 |
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| 中文摘要: |
| 为确定热带海洋环境中琼胶降解菌的最佳产酶条件和最适培养条件，提高其产酶能力，采用3,5-二硝
基水杨酸（3,5-Dinitrosalicylic acid，DNS）比色法测酶活，研究其酶学性质并利用高通量测序获得其参加糖代谢的酶。
单因素和多因素研究结果表明院FG12 的最佳产酶条件为1%蛋白胨、0.8%琼脂、0.02% K2HPO4、pH 7.5、28益；最适培
养条件为0.8%琼脂粉、0.5%蛋白胨、pH 6.8。优化后的酶活达到739.58 U/mL，比优化前提高了273.49%。琼胶酶的最
适温度为36益，在36~55益之间的热稳定性很好；最适pH 值为7.5，在pH 为6.5~7.5 时，酸碱稳定性较好。高通量测
序的代谢图中，FG12 中含有多种不同数量的酶参与糖代谢，能通过基因改造提高酶产量。因此，FG12 是一株高效琼
胶降解菌，为潜在的工程菌。 |
| 英文摘要: |
| To determine the optimal fermentation conditions and culture conditions of FG12, an agarase-producing
strain was taken from the tropical marine environment for improving its enzyme activity, 3,5-Dinitrosalicylic acid (DNS)
method was used to determine the enzyme activity and study its enzymatic properties, and the high-throughput sequencing
was applied to obtain enzymes involved in glucose metabolism. By the test of single factors and multiple factor, the optimal
agarase-producing medium was as follows: 0.8% agar, 1% peptone, 0.02% K2HPO4, 28 and pH 7.5; the optimal culture
medium was as follows: 0.8% agar, 0.5% peptone and pH 6.8. The activity reached the highest value of 739.58 U/mL which
was 273.49% times higher than that under the original conditions. Enzymatic properties showed that its optimal temperature
and pH were 36 and 7.5, respectively, it was stable at 36~55 and pH 6.5~7.5. Analysis on KEGG metabolism pathways
showed that a variety of different enzymes from FG12 were involved in glucose metabolism, which could improve the
enzyme production through genetic modification. The results indicate that FG12, an efficient agarase-producing strain,
might act as a potential candidate for the industrial application. |
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