李晶晶1,2、李烜桢3、张宝2,4、冯法节2,4、曹晓风2,4、李振方2,4、张重义2,4.不同来源地黄化感物质对土壤微生物
功能多样性的影响[J].广东农业科学,2014,41(10):48-50 |
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不同来源地黄化感物质对土壤微生物
功能多样性的影响 |
Effects of allelochemicals from different parts ofRehmannia glutinosa L. on the functionaldiversity of microbial flora in soil |
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DOI: |
中文关键词: 地黄 化感物质 土壤微生物 功能多样性 BIOLOG |
英文关键词: Rehmannia glutinosa L. allelochemical microbial flora in soil functional diversity BIOLOG |
基金项目:国家自然科学基金(31201694、81274022) |
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中文摘要: |
地黄连作障碍严重制约了地黄的产量和品质、化感自毒作用是植物连作障碍形成的重要原因之一、研
究地黄化感物质的来源(根尧叶尧根系分泌物)显得尤为重要。通过在土壤中添加地黄化感物质的不同载体(根尧叶尧根
系分泌物)、对一段时期内土壤微生物功能多样性发生的变化进行分析、基于BIOLOG 平板法分析不同时期各个处
理以酚酸类物质为碳源及以其他碳源为底物的微生物生长情况、研究化感物质的主要来源。结果表明院处理1 d、根
处理最有利于以多聚物尧氨基酸尧糖类尧胺类尧羧酸为碳源的微生物生长、叶处理最有利于以酚酸为碳源的微生物生
长、根系分泌物处理微生物生长情况仅好于对照。处理30尧60尧90尧180 d、根处理最有利于以酚酸为碳源的微生物生
长、叶处理最有利于以多聚物尧氨基酸为碳源的微生物生长、根系分泌物处理微生物多样性最差、不利于以各类碳源
为底物的微生物生长。 |
英文摘要: |
Consecutive monoculture problem has seriously restrained the yield and quality of Rehmannia glutinosa L.
Allelopathic autotoxicity is one of the most important reasons for the formation of plants consecutive monoculture problem,
so it is particularly important to study the source of allelochemicals (root, leaf and root exudates) of R. glutinosa L. The
paper investigated the changes in the functional diversities of microbial flora in soil during different periods after adding
different carriers of allelochemicals from R. glutinosa L. (root, leaf and root exudates) to soil. By BIOLOG technology
analyzing the growth of microbial flora feeding on phenolic acids and other carbohydrates as carbon sources of every
treatments during different periods, the author studied the main source of allelochemicals. BIOLOG analysis results
indicated that, when treated for 1 day, the root treatment was most conductive to the growth of the microbial flora feeding
on polymers, amino acids, amine, carboxylic acids and carbo-hydrates as carbon sources, the leaf treatment was most
conductive to the growth of the microbial flora feeding on phenolic acids as carbon sources, and the root exudates
treatment爷s growth of microbial flora in soil was just better than the control. When treated for 30 60 90 180 days, the
root treatment was best for the growth of the microbial flora feeding on phenolic acids as carbon sources, the leaf treatmen was most beneficial to the growth of microbial flora
feeding on polymers, amino acids as carbon sources,
while the root exudates treatment was not conductive to
the growth of microbial flora feeding on all carbon
sources. |
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