| 程杏安、周晓武、张淑明、蒋旭红、刘展眉.植物甾醇对黑色素瘤细胞的生长
抑制及凋亡诱导作用[J].广东农业科学,2014,41(10):94-97 |
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植物甾醇对黑色素瘤细胞的生长
抑制及凋亡诱导作用 |
| Effects of phytosterol on growth inhibiting andapoptosis induction of melanoma cells |
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| DOI： |
| 中文关键词: 茁-谷甾醇 豆甾醇 B16F10 黑色素瘤细胞 细胞凋亡 |
| 英文关键词: β-sitosterol stigmasterol B16F10 melanoma cell cellular apoptosis |
| 基金项目:国家自然科学基金（21376281）；广东省自然科学
基金（S2013040011746） |
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| 摘要点击次数: 1807 |
| 全文下载次数: 761 |
| 中文摘要: |
| 利用MTT 检测豆甾醇和茁-豆甾醇对B16-F10 黑色素瘤细胞活力的影响、同时通过倒置生物显微镜和
荧光显微技术观察两种植物甾醇对B16F10 细胞形态特征的影响。结果表明、茁-谷甾醇（0.20、0.30 mmol/L）能够明显
抑制B16F10 细胞的生长、并总体上表现出一定的浓度依赖性。豆甾醇（0.20、0.25 mmol/L）处理B16-F10 细胞、细胞
生长受到明显抑制、但浓度依赖性不明显。同时显微观察发现茁-谷甾醇和豆甾醇对B16-F10 细胞均具有明显的凋
亡诱导作用、豆甾醇处理48~72 h 细胞出现大量凋亡小体、贴壁细胞逐渐减少、漂浮死亡细胞逐渐增多、表现出凋亡
的典型特征；茁-谷甾醇处理组细胞以空泡化为主、也出现相当数量的凋亡小体。DAPI 染色观察表明茁-谷甾醇和豆
甾醇处理72 h、大量细胞出现染色质凝聚、细胞核膨大或形成凋亡小体等凋亡现象。综合结果表明、茁-谷甾醇和豆甾
醇一定程度上通过诱导细胞凋亡、抑制B16F10 黑色素瘤细胞的生长。 |
| 英文摘要: |
| Effects on viability and morphology changes of melanoma B16F10 cells induced by stigmasterol and 茁-
sitosterol were studied by MTT, inverted phase microscope and fluorescence microscopy. The results indicated that 茁-
sitosterol (0.20, 0.30 mmol/L) inhibited the growth of B16F10 cells and significantly showed dose-dependence. Stigmasterol
(0.20, 0.25 mmol/L) also inhibited the growth of B16F10, but showed no dose-dependence. Meanwhile, significant apoptosis
was induced by stigmasterol and 茁-sitosterol. Identified by the increase of apoptotic bodies and death cells, and the
decrease of adherent cells, typical apoptosis appeared in B16F10 cells treated by stigmasterol for 48-72 h. A number of
cells in presence of 茁-sitosterol appeared vacuoles and some parts of cells appeared apoptotic bodies. Fluorescence
microscopy established that numbers of cells treated by stigmasterol and 茁-sitosterol appeared apoptotic characteristics,
like condensed chromosome, swollen nuclear and apoptotic body. Accordingly, stigmasterol and 茁-sitosterol partly inhibited
B16F10 cells growth through inducing the cells apoptosis. |
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