| 李雅韵1,2、廖文彬2、王斌2、杨义伶2、郭鑫1,2、杨子1,2、彭明2.干旱胁迫下木薯AP2转录因子的
荧光定量PCR 分析[J].广东农业科学,2014,41(10):126-131 |
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干旱胁迫下木薯AP2转录因子的
荧光定量PCR 分析 |
| AP2 transcription factor expression in cassava under droughtstress by real-time fluorescent quantitative PCR |
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| DOI： |
| 中文关键词: 干旱胁迫 AP2转录因子 荧光定量PCR 基因表达 |
| 英文关键词: drought stress AP2 transcription factor real-time fluorescent quantitative PCR gene expression |
| 基金项目:国家国际科技合作专项(2013DFA32020)；国家
野863冶计划项目(2012AA101204-2)；国家野973冶计划项目(2010C
B126600)；海南省科技厅重点项目(ZDZX2013023 |
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| 摘要点击次数: 1363 |
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| 中文摘要: |
| 为研究木薯的两个AP2基因在干旱胁迫下的表达模式、对木薯品种华南5 号进行干旱胁迫处理、提取
各个时间点离区部位的RNA、以其反转录的cDNA 为模板、应用SYBR Green玉荧光定量PCR 检测AP2转录因子基
因的差异性表达。结果表明、目的基因的熔解曲线都只出现一个峰、说明引物特异性强、且各基因的扩增效率与参
照基因Actin 接近、试验结果可用2-驻驻CT 计算法进行分析。经分析、两个AP2家族成员在干旱胁迫下均诱导表达、其
表达模式不完全相同且存在一定的相关性、这与之前做过的基因芯片杂交结果相符。 |
| 英文摘要: |
| In order to study the expression patterns of two AP2 genes in cassava under drought stress, the cassava
variety SC5 was treated with drought stress and RNA was extracted from the leaf abscission zone of different time points.
We detected the different expression of AP2 transcription factors by SYBR Green玉real-time fluorescent quantitative PCR
with the cDNA obtained from reverse transcription as templates. The results showed that melting curves of both target genes
had only one peak, indicating that the primers were specific and the amplification efficiency of target genes were close to
reference gene Actin. The experimental results could be analyzed by 2-驻驻CT calculation method. After analysis, the result
showed that the two AP2 family members were induced under drought stress, the expression patterns were not identical and
there was certain correlation. These results were consistent with microarray hybridization results. |
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