| 李兴涵1、2、费小雯3、邓晓东2.莱茵衣藻磷酸果糖激酶RNAi载体构建
及其对莱茵衣藻油脂积累的影响[J].广东农业科学,2014,41(10):155-159 |
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莱茵衣藻磷酸果糖激酶RNAi载体构建
及其对莱茵衣藻油脂积累的影响 |
| Construction of phosphofructokinase RNAi vectorsand its impact on lipid accumulationin Chlamydomonas reinhardtii |
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| DOI： |
| 中文关键词: 莱茵衣藻 磷酸果糖激酶 RNAi 油脂含量 |
| 英文关键词: Chlamydomonas reinhardtii phosphofructokinase RNAi oil content |
| 基金项目:国家自然科学基金（30960032；31000117）；海南
省重大科技项目（ZDZX2013023-1）；中央公益；所基本业务费
（CATAS-ITBB110507、130305）；中央公益；所基本业务费热科
；本级（1630052013009）；海南省自然科学基金（313077） |
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| 中文摘要: |
| 为研究磷酸果糖激酶（PFK2）对微藻油脂积累的影响、克隆了莱茵衣藻磷酸果糖激酶同源基因CrPFK2、
构建CrPFK2 RNAi 干涉载体并转化莱茵衣藻。通过测定转基因藻在HSM 培养下的生物量和油脂含量的结果发现、
CrPFK2 RNAi 转基因藻株在HSM 培养基中生长加快、油脂含量下降了17.03%耀21.48%、说明CrPFK2 基因表达的降
低与藻细胞油脂含量减少成正相关。CrPFK2 通过改变光合碳流的多少间接调控藻细胞油脂的合成。该结果为
CrPFK2 基因应用于微藻油脂的遗传改良将起到重要作用。 |
| 英文摘要: |
| To study effects of PFK2 on lipid accumulation in microalgae, the phosphofructokinase homologous gene 2
CrPFK2 was cloned, a CrPFK2 RNAi interference vector was constructed and transferred into Chlamydomonas reinhardtii.
Measured the biomass and lipid content of the transgenic algal, the growth rate of transgenic algae strain showed an
increase in the HSM medium, while it had a decrease in the oil content (by 17.03%-21.48%). The results indicated that
the downstream of CrPFK2 expression positively regulated the oil content of the algal cells and CrPFK2 indirectly
controlled the oil synthesis of algal cells by changing the photosynthetic carbon flow. These results suggest that CrPFK2
may play an important role in the genetic improvement of microalgae oil. |
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