| 贵祥1，曾涛2,3，郭素霞3，陈汉清2，曾会才2,3.香蕉枯萎病4号小种T-DNA 插入突变体
Focr4-1453表型特征及致病性分析[J].广东农业科学,2014,41(12):85-88 |
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香蕉枯萎病4号小种T-DNA 插入突变体
Focr4-1453表型特征及致病性分析 |
| Phenotype and pathogenicity of Focr4-1453院T-DNA insertionmutant of Fusarium oxysporum f. sp. cubense race 4 |
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| DOI： |
| 中文关键词: 香蕉枯萎病 4 号生理小种 致病性测定 生物学表型 |
| 英文关键词: Fusarium wilt of Banana Fusarium oxysporum f. sp. cubense race 4 pathogenicity test biology phenotype |
| 基金项目:国家公益性行业(农业）科研专项(200903049）；
国家自然科学基金(31271995） |
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| 摘要点击次数: 1638 |
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| 中文摘要: |
| 尖孢镰刀菌古巴转化型是引起香蕉枯萎病的主要病原菌，呈世界多态性分布，其致病机理尚不清楚。
Focr4-1453 为T-DNA 插入突变体库中筛选出的一株与致病性相关的突变体。在克隆出其失活基因之后，将“生型
菌株中的相同基因进行敲除和互补，得到敲除突变体驻Foc4-1453 和互补突变体驻Foc4-1453-cp-1。对获得的3 株
突变体进行生物学表型研究发现，这些菌株的菌落生长速度、产孢能力以及孢子萌发率较“生型菌株明显降低，且
无法透过玻璃纸进行生长。致病性测定试验表明，该基因失活会导致病原菌侵染能力显著减弱。 |
| 英文摘要: |
| Banana fusarium-wilt disease was caused by Fusarium oxysporum f.sp. cubense and it爷t pathogenesis is
unclear. Focr4-1453 is a pathogenicity-related mutant strain and it is found from T-DNA library. We got the mutant gene
cDNA full length, then knockouted and complemented this mutant gene from wild-type strain and named them 驻Foc4-
1453 and 驻Foc4-1453-cp -1. The result showed that the biological phenotype of Focr4-1453爷驻Foc4-1453 and 驻Foc4-
1453-cp -1 changed. Their colony growth rates, spore production capacity and spore germination were lower than those of
wild-type strain. In addition, their virulence significantly reduced. |
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