| 李欢欢1，蔡俊鹏1,2.蛭弧菌对九孔鲍生长及养殖
体系细菌群落的影响[J].广东农业科学,2014,41(12):127-132 |
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蛭弧菌对九孔鲍生长及养殖
体系细菌群落的影响 |
| Effects of Bdellovibrio-and-like organism on growthof Haliotis diversicolor aquatilis and bacterialcommunity in rearing system |
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| DOI： |
| 中文关键词: 蛭弧菌 九孔鲍 弧菌 微生物群落数 PCR-DGGE(变性梯度凝胶电泳） |
| 英文关键词: Bdellovibrio-and-like organism Haliotis diversicolor aquatilis Vibrio bacterial community PCR-DGGE
(PCR amplified 16S rDNA-denaturing gradient gel electrophoresis) |
| 基金项目: |
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| 摘要点击次数: 1739 |
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| 中文摘要: |
| 研究了蛭弧菌BDH12(登录号为EF094471）对九孔鲍(Haliotis diversicolor aquatilis) 生长、肠道和养殖水
体总菌、弧菌以及水体细菌群落数的影响。结果显示，蛭弧菌组九孔鲍的存活率、壳长和体重分别比对照组增加了
111%、1.8%和7.0%；蛭弧菌组的九孔鲍肠道总菌和弧菌分别比对照组降低8.6%~21.0%和12.2%~27.7%，养殖水体总
菌和弧菌分别比对照组降低2.1%~16.7%和8.0%~34.8%；养殖水体的PCR-DGGE 图谱显示，对照组和蛭弧菌组养殖
水体的细菌丰度值分别在0.54~0.97 和0.50~0.75 之间。结果表明，蛭弧菌BDH12 可以作为一种生物控制因子用于
九孔鲍的健康养殖。 |
| 英文摘要: |
| In this study, Bdellovibrio -and-like organism strain BDH12 (GenBank accession No. EF094471) was
applied in a 63-day rearing of Haliotis diversicolor aquatilis, and its effects on abalone growth, the total number of
bacterial and Vibrio both in rearing water and abalone intestine and community structure of rearing water were investigated.
The results showed that the survival rate, abalone length and weight of BDH12 added groups significantly increased by
111%, 1.8% and 7.0%, respectively, compared with the control. The total number of bacterial and Vibrio of BDH12 added
tanks in abalone intestine significantly reduced by 8.6%-21.0% and 12.2%-27.7%, and in water they reduced by 2.1%-
16.7% and 8.0%-34.8%, respectively, compared with the control. PCR-DGGE profiles revealed that the richness value of
bacterial communities of the control and the test groups were 0.54-0.97 and 0.50-0.75. The results indicate that BDH12
can be used as a bio-control/probiotic agent in H. diversicolor aquatilis culture. |
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