卢晶霞1,2,杨松光1,吴克强1.番茄组蛋白去乙酰化酶HD2家族生物
信息学及表达模式分析[J].广东农业科学,2014,41(12):138-143 |
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番茄组蛋白去乙酰化酶HD2家族生物
信息学及表达模式分析 |
Bioinformatics and expression pattern analysis of Histonedeacetylase HD2 subfamily in tomato (Solanum lycopersicum) |
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DOI: |
中文关键词: 组蛋白去乙酰化 生物信息学分析 表达模式 组蛋白修饰 |
英文关键词: histone deacetylase bioinformatic analysis expression pattern histone modification |
基金项目:国家重点基础研究发展计划项目(2012CB910902);
国家自然科学基金面上项目(31371308) |
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中文摘要: |
在生物信息学分析和克隆番茄组蛋白去乙酰化酶HD2 亚家族的基础上,探究了HD2 家族在番茄果实
发育阶段的表达模式。结果显示,番茄HD2 家族有3 个成员,其氨基酸序N 端具有典型的MEFWG 五肽基序,中部
为疏水结构域分隔开的两段富含酸性氨基酸的结构域,C 端的序列呈多样性,并且具有C2H2 型锌指结构;亚细胞定
位预测发现该家族成员均定位于细胞核,这与其参与组蛋白修饰功能相一致。表达模式结果显示,HD2 基因家族在
果实发育阶段中具有阶段特异性,推测其在调控番茄的果实发育方面有重要作用。 |
英文摘要: |
Histone acetylation plays a key role in regulating chromatin organization and gene expression. In this study,
we successfully identified and cloned three tomato HD2 genes, SlHDT1101, SlHDT1102 and SlHDT1103. All the three
tomato HD2 proteins had a characteristic domain structurea conserved pentapeptide motif (MEFWG) on the N-terminal
and two highly charged acidic stretches in the central part. In addition, both SlHDT1101 and SlHDT1103 contained a
C2H2 type zinc finger in the C terminal. Furthermore, all the three HD2 proteins were localized in the nuclear. The gene
expression analysis showed that each of the three HD2 genes displayed a specific expression pattern in tomato fruit
development, indicating their important functions during fruit development. |
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