| 何立美，陈玉婷，刘洪梅，彭新宇，潘育方，袁明贵，罗胜军，魏光伟.高效液相色谱法同时测定水辣蓼提取物中6种黄酮成分的含量[J].广东农业科学,2014,41(13):94-98 |
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高效液相色谱法同时测定水辣蓼提取物中6种黄酮成分的含量 |
| Simultaneous determination of six 噪蚤灶凿泽燥枣flavonoid in extract of Polygonum hydropiper L. by HPLC |
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| DOI： |
| 中文关键词: 辣蓼 黄酮 高效液相色谱 含量测定 |
| 英文关键词: Polygonum hydropiper L. flavonoid HPLC content determination |
| 基金项目:国家公益性行业（农业）科研专项（201303040-16） |
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| 摘要点击次数: 1847 |
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| 中文摘要: |
| 建立了同时定量检测水辣蓼中6种黄酮类成分的高效液相色谱方法：选择Waters Symmetry shield RP18（4.6 mm×250 mm, 5.0 μm）色谱柱，流动相甲醇-0.4%甲酸水溶液（梯度洗脱），流速1 mL/min，柱温30℃，检测波长359nm，进样量10μL。结果表明，6种黄酮成分在50min内达到基线分离，在0.1~20μg/mL 浓度范围内，6种黄酮成分的线性相关系数R²≥0.9995，平均加样回收率为96.65%~99.54%，RSD为0.82%~1.38%（n=3）。该方法准确、简便，重复性好，可用于水辣蓼中芦丁、金丝桃苷、槲皮苷、槲皮素、异鼠李素和山奈酚的含量测定，为控制水辣蓼药材的质量提供依据。 |
| 英文摘要: |
| This paper established a method of quantitative detection for six kinds of flavonoid content in extract of Polygonum hydropiper L. by high performance liquid chromatography (HPLC). The analysis was performed on a Waters Symmetry shield RP18 (4.6 mm伊250 mm, 5.0 滋m) column. Mobile phase was methanol-0.4% formic acid aqueous solution (gradient elution). The flow rate was set at 1.0 mL/min and the eluent was detected at 359 nm at 30益. The calibration curves of the six kinds of flavonoid were linear(R2>0.9995) over the concentration range of 0.1~20 滋g/mL. The mean recoveries were 96.65% to 99.54%, RSD was 0.82%~1.38% (n=3). The results indicate that the method is accurate and simple, has good repeatability, can apply to simultaneous determination of Rutin, Hyperoside, Quercitrin, Quercetin, Isorhamnetin and Kaempferol in the extract of P. hydropiper L., to provide the basis for control the quality of P. hydropiper L. |
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