| 王瑛华，陈刚，陈宁，陈雄伟，曾晓霞.肇庆柑橘种质遗传多样性的RAPD分析[J].广东农业科学,2014,41(14):126-129 |
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肇庆柑橘种质遗传多样性的RAPD分析 |
| Genetic diversity analysis of Citrus reticulatacultured in Zhaoqing based on RAPD |
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| DOI： |
| 中文关键词: 柑橘 肇庆 RAPD 亲缘性分析 |
| 英文关键词: Citrus reticulata Zhaoqing RAPD phylogenetic analysis |
| 基金项目:广东省教育部产学研结合项目（2010B09040037）；广东省科技计划项目（2011B020417001）；肇庆学院第四轮重点建设学科（植物学）专项（2012） |
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| 中文摘要: |
| 采用RAPD分子标记技术对广东省肇庆地区5个柑橘栽培品种进行遗传多样性分析。结果表明：从60个随机引物中筛选出40个重复性好、稳定性高的有效引物，共扩增出459条带，其中多态性条带为309条，多态性比例为67.32%。5个供试品种间的遗传相似系数范围为0.33~0.74。UPGMA聚类分析结果表明，在遗传相似系数为0.41时，5个栽培品种可分为2个类群：马水橘独自为1类曰其他4个为1类；当遗传相似系数为0.55时，贡柑单独为1小类，砂糖橘、八月橘和酸橘为1小类。 |
| 英文摘要: |
| Genetic diversity of 5 Citrus accessions cultivated in Zhaoqing Area of Guangdong Province, was assessed with random amplified polymorphic DNA (RAPD) markers. 40 form 60 arbitrary primers were selected, which could well generated amplication products repeatably and stably. Using these 40 primers, a total of 459 bands were identified, 309 of which were polymorphic, corresponding to 67.32% of the amplification products. The genetic similarity coefficient (GSC) of paired varieties ranged from 0.33 to 0.74. Cluster analysis was conducted using UPGMA. The result showed the 5 Citrus accessions could be divided into two groups when GSC was 0.41, the group Ⅰcontained Fortunella margarita (Lour.) Swingle alone, while the group Ⅱwas comprised of the other four Citrus accessions. When GSC was 0.55 Citrus reticulata Blanco var. gonggan was the only member of subgroup I, the subgroup Ⅱwas consisted of C. reticulata ‘Shangtangju’, C. reticulata Blanco cv.‘Bayueju’and C. sunki Hort. ex Tanaka. |
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