| 刘汉斌，彭娇娇，魏文康，翟少伦，熊惠军，吕殿红.强鸡散对免疫抑制小鼠脾转录因子GATA-3和T-bet 表达量的影响[J].广东农业科学,2014,41(19):122-125 |
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强鸡散对免疫抑制小鼠脾转录因子GATA-3和T-bet 表达量的影响 |
| Effect of strong chicken powder on relative expression of GATA-3 and T-bet mRNA in mice spleen immunosuppressed by cyclophosphamide |
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| DOI： |
| 中文关键词: 强鸡散 荧光定量PCR GATA-3 T-bet |
| 英文关键词: strong chicken powder fluorescence quantitative PCR GATA-3 T-bet |
| 基金项目:广东省科技计划项目(2010B031000016)；科技部星火计划项目（2012GA780002） |
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| 中文摘要: |
| 试验旨在通过定量检测小鼠转录因子T-bet、GATA-3 mRNA 表达水平，探讨强鸡散对环磷酰胺致免疫抑制小鼠转录因子GATA-3 和T-bet 的调节作用。40只体重20g左右的昆明纯种小鼠被随机分为4组：环磷酰胺组、中药组、环磷酰胺+中药组、对照组，采用实时荧光定量PCR方法，以 β-actin 基因作为内参基因，检测T-bet 和GATA-3 mRNA 的表达量。结果显示：强鸡散能改变小鼠脾脏转录因子GATA-3 的相对表达量，对T-bet基因表达量没有明显影响。表明强鸡散具有调节环磷酰胺致免疫抑制小鼠脾脏转录因子GATA-3 和T-bet 表达量的作用，通过降低GATA-3 的表达量，间接减少Th2 细胞的生成，使Th1/Th2 平衡向Th1方向转化。 |
| 英文摘要: |
| This study was designed to investigate the mRNA expressions of GATA-3 and T-bet of spleen in tested mice after immunosuppressed by cyclophosphamide, then intended to understand the regulation mRNA expression levels of GATA-3 and T-bet. 40 purebred ICR mice were divided into four groups randomly, including cyclophosphamide group,Chinese medicine group, cyclophosphamide and Chinese medicine group, the normal control group. Using fluorescent quantitative PCR, the GATA-3 mRNA and T-bet expression in spleen were investigated in the four groups, taking β-actin gene as a reference gene. The results suggest that strong chicken powder can regulate immune function through relieving immunosuppressive effects caused by cyclophosphamide and improving immune function inhibition. |
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