| 许云，汪倩，易太鲜，赵同建.一株产纤维素酶细菌的分离鉴定及产酶条件初探[J].广东农业科学,2014,41(19):150-153 |
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一株产纤维素酶细菌的分离鉴定及产酶条件初探 |
| Isolation and identification of a cellulase-producing bacteria and preliminary study on its fermentation conditions |
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| DOI： |
| 中文关键词: 纤维素酶 鉴定 产酶条件 |
| 英文关键词: cellulase identification fermentation condition |
| 基金项目:海南省自然科学基金（310024）；海南省教育厅高等学校科研项目（Hjkj2010-06） |
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| 中文摘要: |
| 对从海南大学薯蓣种质资源圃的不同大薯根部土壤中分离的产纤维素酶细菌X-6 进行鉴定及产酶条件研究。采用细胞学染色、生理生化指标、16S rRNA 基因序列同源性分析测定等方法对菌株进行鉴定，并采用正交试验研究培养时间、温度、pH 值和碳氮比等因素对菌株产纤维素酶活力的影响，初步确定其产纤维素酶的最优条件组合。菌株X-6 鉴定为蜡状芽孢杆菌、革兰氏阳性菌、菌株芽孢着生在细菌中部位置，淀粉酶、明胶水解酶阳性。GenBank 中X-6 菌株的16S rRNA 基因序列与HQ844479 Bacillus cereus strain JSYM28 相似性最高（99%）。菌株的最优培养条件为：培养基碳氮比为2：4，pH 7.0，42℃培养72 h，在此条件下CMC 酶活力和滤纸酶活力分别为137.10、171.07 U/mL。 |
| 英文摘要: |
| Cytological staining, physiological and biochemical characteristics, and 16S rRNA gene sequences analysis were utilized to identify cellulase-producing bacteria X-6, which was isolated from root soils of the different water yams in Yam Germplasm Resources of Hainan University. Then orthogonal experiment designs were applied to study the effects of incubation time, temperature, pH and ratio of carbon-nitrogen on the cellulase-producing enzyme activity of X-6. The results showed that the strain X-6 was identified as Bacillus cereus, with Gram-positive, amylase-positive, gelatinasepositive and spore in the middle of the bacterium cell. Its 16S rRNA gene was highly similar to Bacillus cereus JSYM28,and the similarity was 99%. The optimal fermentation conditions were as following value of pH was 7.0, the ratio of carbon-nitrogen was 2：4, the temperature was 42℃ for 72 h. Under the optimal fermentation conditions, CMC and FPA enzyme activity were respectively 137.10 U/mL and 171.07 U/mL. |
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