| 丁栩，李琴，张驰.紫油厚朴中硒多糖的提取工艺条件优化[J].广东农业科学,2014,41(20):104-106 |
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紫油厚朴中硒多糖的提取工艺条件优化 |
| Optimization on the extraction of Se-polysaccharides from Magnolia officinalis by orthogonal test |
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| DOI： |
| 中文关键词: 紫油厚朴 硒多糖 正交试验 工艺优化 |
| 英文关键词: Magnolia officinalis Rehd. et Wils Se-polysaccharide orthogonal experiment process optimization |
| 基金项目:国家自然科学基金（61263030/F030304）；湖北省科技厅重点基金（2011CDA011）；生物资源保护与利用湖北省重点实验室开放基金（2011）；湖北省大学科技创新项目（201310517016） |
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| 中文摘要: |
| 为优选恩施富硒紫油厚朴中硒多糖提取分离的最佳工艺，以恩施高硒区所产的富硒厚朴为材料，采用正交试验法水提醇沉得到含硒多糖，用硫酸-苯酚法测定多糖含量，原子荧光法检测硒含量，以多糖量为指标，优选料液比、微波处理时间、提取时间对硒多糖提取的影响。结果表明，恩施高硒厚朴内含硒多糖的最佳提取分离条件为料液比1：100、微波处理4min，浸提90min，在此条件下提取率为2.022%，多糖中硒含量为0.1305 μg/g。 |
| 英文摘要: |
| In order to select the optimal process conditions for extracting Se-polysaccharide from Magnolia officinalis,we conduct the experiment on the Magnolia officinalis growing in the high - selenium area of Enshi. We get the polysaccharide through water extraction and alcohol precipitation using orthogonal experimental design, determine the polysaccharide content by sulfuric acid-phenol method, detect the selenium content by atomic fluorescence spectrometry,and set the amount of polysaccharide as the index to explore the influence of the following conditions: raw material -liquid ratio, microwave processing time and extraction time. The result shows that the optimal conditions for polysaccharide extraction are as follow: raw material -liquid ratio set to 1：100, 4 min microwave treatment, 90 min extraction. The extraction rate should be 2.022% and Selenium content of polysaccharides can be 0.1305 μg/g under the extraction conditions described above. |
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