| 万鹏,任南南,蔡冰娜,陈华,陈得科,孙恢礼,潘剑宇.香港巨牡蛎中牛磺酸的超声、酶解提取工艺研究[J].广东农业科学,2014,41(20):114-118 |
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香港巨牡蛎中牛磺酸的超声、酶解提取工艺研究 |
| Study on extraction technique of taurine from Crassostrea hongkongensis with ultrasonic and enzymatic hydrolysis |
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| DOI： |
| 中文关键词: 香港巨牡蛎 牛磺酸 超声 酶解 提取 |
| 英文关键词: Crassostrea hongkongensis taurine ultrasonic enzymatic hydrolysis extraction |
| 基金项目:"十二五"农村领域国家科技计划项目(2012BAD33B10-3);国家海洋局海洋公益性行业科研专项(201305018-2);广东省海洋区域经济创新示范项目(SZHY2012-B01-004);广东省海洋经济创新发展区域专项(GD2013-B03-001);国家自然科学基金青年基金(31101271);广东省中国科学院全面战略合作项目( 2011B090300057,2012B091000025);中国科学院南海海洋研究所青年人才领域前沿项目(SQ201017);广东省佛山市院市合作项目(2012HY100241) |
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| 中文摘要: |
| 以牛磺酸得率为考察指标,通过单因素和正交试验,研究了超声法、蛋白酶酶解法提取香港巨牡蛎中牛磺酸最佳工艺条件，并在最优工艺条件下进行超声辅助酶解提取。确定了超声法最佳工艺条件为超声时间40 min、功率825 W、温度70℃，牛磺酸得率为0.276 （±0.013）%；碱性蛋白酶作为优选酶种，最佳工艺条件为加酶量4000U/g、料液比1：2、酶解时间6h，牛磺酸得率为0.746 （±0.053）%，显著高于超声提取法；超声辅助酶解提取，牛磺酸得率为0.782 （±0.023）%，高于碱性蛋白酶酶解法，可优选应用于牡蛎牛磺酸的提取. |
| 英文摘要: |
| In this paper, single-factor test combined with orthogonal test were used to study the optimum condition of ultrasonic extraction and enzymatic hydrolysis on taurine from Crassostrea hongkongensis, and the ultrasonic -assisted enzymatic hydrolysis under the optimal condition was investigated simultaneously, in which the taurine yield was used as
the indexes. The optimized conditions for ultrasonic extraction were obtained as extraction time of 40 min, ultrasonic power of 825 W, extraction temperature of 70℃ and the taurine yield was 0.276（±0.013)%. The alkaline protease was selected as the optimum enzyme and the optimum condition was obtained as enzyme amount of 4 000 U/g, ratio of material to solvent of 1:2 (g/mL), hydrolysis time of 6 h and the taurine yield was 0.746 (±0.053)%, which was significantly higher than that of ultrasonic extraction. The taurie yield of ultrasonic-assisted enzymatic hydrolysis (0.782 ±0.023%) was higher than that of alkaline protease hydrolysis, which indicated that ultrasonic -assisted enzymatic hydrolysis could be an effective technique for oyster taurine extraction. |
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