| 龚俞，杨忠诚，杨永强，刘若余.威宁黄牛STAM1基因SNPs及其与生长性状关联研究[J].广东农业科学,2014,41(20):136-140 |
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威宁黄牛STAM1基因SNPs及其与生长性状关联研究 |
| Study on the association of polymorphisms in STAM1 gene with growth traits of Weining cattle |
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| DOI： |
| 中文关键词: STAM1 SNP 外显子 内含子 威宁黄牛 |
| 英文关键词: STAM1 SNP exon intron Weining cattle |
| 基金项目:贵州省重大科技专项（黔科合重大专项字[2011]6009号） |
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| 中文摘要: |
| 为研究STAM1 基因多态性与贵州地方黄牛品种威宁黄牛生长性状关联性，选择106 头威宁黄牛为对象，利用PCR-SSCP、DNA测序方法分析STAM1基因SNP与不同个体生长性状的相关性。结果表明：在STAM1基因第3内含子区存在1个SNP 位点，命名为G+31122A。G+31122A位点在威宁黄牛中有不同分型，在威宁黄牛公畜中，G+31122A 位点与胸围、坐骨端宽极显著相关，AA型个体胸围均值极显著低于GA型和GG型个体。而在母畜中，该位点对体直长的影响达到显著性水平，GG型个体体直长均值显著高于AA型个体。说明G+31122A 突变与威宁黄牛生长性状有一定关联。 |
| 英文摘要: |
| In order to study the correlation between the SNPs of STAM1 gene and growth traits in Guizhou local cattle,a total of 106 Weining cattle were used in this study, moreover, PCR-SSCP and DNA sequencing methods were used to analyze the association between SNP and specific growth traits. Results showed that there was only one SNP site existed in the third intron region of STAM1 gene which named G+31122A, furthermore, various genotypes existed in different animals for the SNP of G+31122A. For the male cattle populations, G+31122A had highly significant effects on chest circumference and hucklebone width (P<0.01), the individuals with homozygote genotype AA had significantly lower mean value than those with GA and GG genotypes. Additionally, for the female cattle populations, G+31122A had significant effect on long body straight (P<0.05) and the mean values of GG genotype animals were higher than those of AA genotype individuals. It indicated that G+31122A has directly correlation with the growth performance of Weining cattle. |
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