| 巫兴东，赵超，周欣，吴明开.流苏石斛多糖提取工艺及不同产地体外抗氧化活性研究[J].广东农业科学,2014,41(21):97-101 |
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流苏石斛多糖提取工艺及不同产地体外抗氧化活性研究 |
| Optimization of extraction technology and in vitro antioxidant activity of Dendrobium fimbriatum polysaccharides from different regions |
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| DOI： |
| 中文关键词: 流苏石斛多糖 提取工艺 响应面法 抗氧化 |
| 英文关键词: Dendrobium fimbriatum polysaccharides extraction technology response surface methodology antioxidant activity |
| 基金项目:贵州省教育厅特色重点实验室建设项目(黔科合KY[2012]005号);贵州省中药材现代产业技术体系建设专项(GZCYTX-02);贵州省发展改革委黔发改高技项目([2013]2068);贵州省中药现代化科技专项(黔科合中药字[2011]5037号) |
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| 中文摘要: |
| 采用响应面法对热浸法提取流苏石斛多糖提取工艺进行优化，并以清除1,1-二苯基-2-三硝基苯肼（DPPH）自由基能力为评价指标，对不同产地流苏石斛多糖抗氧化活性进行比较研究。结果表明:流苏石斛多糖提取最佳工艺参数为提取温度92℃、料液比（V/W）26：1、提取时间2.5 h，理论提取率为9.31%，实际提取率为9.18%，相对误差为1.40%；兴义、罗甸、云南、广西4个产地的流苏石斛多糖含量分别为10.34%、8.36%、3.35%、6.66%；以IC50作为判定抗氧化活性强弱的指标，分析表明，4个不同产地中以云南产流苏石斛多糖抗氧化能力相对较强。 |
| 英文摘要: |
| The extraction technology of polysaccharides from Dendrobium fimbriatum by reflux method was optimized
by using the response surface method. The antioxidant activities of D. fimbriatum polysaccharides collected from different
regions in China were investigated using in vitro antioxidant assays: 1,1 -Diphenyl -2 -picrylhydrazyl (DPPH) radical
scavenging activities. The results showed that the optimal extraction conditions were as follows: extraction temperature of 92℃, ratio of material to solvent of 1:26 and extraction time of 2.5 h. Under these conditions, the yield of D. fimbriatum polysaccharides was up to 9.18%, the best predicative extraction rate of the polysaccharides was 9.31%, and the relative error was 1.40%. The contents of polysaccharides in D. fimbriatum from Xingyi Guizhou, Luodian Guizhou, Yunnan,
Guangxi were 10.34%, 8.36%, 3.35% and 6.66%, respectively. Among these 4 samples, the sample collected from Yunnan
had the relatively higher antioxidant activity by using IC50 value as an index. |
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