| 刘燕，胡向丹，马莹，李鑫.一株降解水杨酸细菌的分离、鉴定及其降解效果[J].广东农业科学,2014,41(22):70-74 |
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一株降解水杨酸细菌的分离、鉴定及其降解效果 |
| Isolation, identification and degradation effect of one SA-degrading bacteria |
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| DOI： |
| 中文关键词: 水杨酸 生物降解 寡养单胞菌 16SrDNA |
| 英文关键词: Ortho-hydroxybenzoic acid biodegradation Stenotrophomonas sp. 16SrDNA |
| 基金项目:贵州省烟草专卖局科技项目（黔烟科[2011]02号） |
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| 摘要点击次数: 1270 |
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| 中文摘要: |
| 以水杨酸为唯一碳源，采用逐步富集培养方法，从贵州省烟草科学院福泉基地烤烟连作土壤中分离得到一株水杨酸降解菌X6。根据形态观察、生理生化鉴定和16SrDNA 序列分析结果，将降解菌株X6鉴定为寡养单胞菌属（Stenotrophomonas sp.）。采用摇床震荡培养方法研究该菌株生理生化特性及其对水杨酸降解效果的影响。结果表明院降解菌X6在30℃震荡培养7d后，在300~400 mg/L 水杨酸浓度范围下降解量和降解率达到最大，分别为63.2mg、18.6%，在400mg/L底物浓度时降解菌生长量达到最大，且该底物浓度下在培养6 d后降解速率达到最大（18.5mg/d）。该菌在植烟土壤修复和烤烟连作上可能具有应用前景。 |
| 英文摘要: |
| By using selective enrichment culture with SA as the sole carbon source, a ortho-hydroxybenzoic aciddegrading
bacterial strain X6 was isolated from the continuous cropping of flue-cured tobacco soil in Fuquan Area of Guizhou Provience Tobacco Institute. The stain was identified as Stenotrophomonas sp., based on its morphological,
physiological, and biochemical characteristics, and similarity identification of 16S rDNA sequence. The physiological -
biochemical characteristic and the effects on the degradation of the strain were investigated by shaking flask culture. After shaking culture with the initial concentration of SA being 300 mg/L-400 mg/L at 30℃for 7 days, the degradation rate and degradation quantity of SA were 18.6% and 63.2 mg. Adding 400 mg/L of consistence, the mount of SA-degrading bacterial
strain X6 was ran up to the most, and the its degradation rate reached the fastest by 18.5 mg/d in the sixth day. The
bacteria has a potential application prospect in the planting tobacco soil remediation and continuous cropping of flue-cured tobacco. |
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