| 王琼,谭志琼,张荣意.野生红花隔距兰根部内生真菌研究[J].广东农业科学,2014,41(22):79-82 |
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野生红花隔距兰根部内生真菌研究 |
| Endophytic fungi in root of wild Cleisostoma williiamsonii |
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| DOI： |
| 中文关键词: 红花隔距兰 内生真菌 分离 鉴定 |
| 英文关键词: Cleisostoma williiamsonii endophyte isolation identification |
| 基金项目:海南大学热带作物种质资源保护与开发利用教育部重点实验室开放基金（2011hckled-05）；海南大学“211工程”建设项目；海南大学环境与植物保护学院研究生创新平台 |
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| 中文摘要: |
| 为了解内生真菌在红花隔距兰根部的种类多样性以及受生境的影响,采用组织培养法,对海南霸王岭地区不同生境下的野生红花隔距兰根部进行内生真菌分离与鉴定。结果共分离到内生真菌145株，确定为15属，其中子囊菌门的Penicillium16株，Aspergillus12株、Pestalotiopsis5株、Fusarium19株、Neonectria2株、Chaetomium4株、Calonectria5株、Xylaria5株，半知菌类的Paecilomyces7株、Alternaria2株、Phomopsis 8株、Colletotrichum32株，接合菌门的Rhizopus5 株、Mucor7 株, 卵菌门的Albugo 6株，有10株菌经ITS序列比对分别与Botryosphaeria sp.、Beltraniella sp.相似度较低，有待进一步鉴定研究确定其分类地位。发现Colletotrichum sp. 、Penicillium sp. 、Fusariumsp.为优势真菌，生在于热带针叶林的植株根部分得的内生真菌最多，生物多样性最丰富。 |
| 英文摘要: |
| As an epiphytic orchid, Cleisostoma williiamsonii is a very beautiful ornamental plant, and plays an important role in tropical forest ecosystems. In this paper, tissue culture method was used to isolate endophyte in root of wild C. williiamsonii from Bawangling, Hainan. The total 145 isolates were identified into 15 genera, including Penicillium 16 strains, Aspergillus 12 strains, Pestalotiopsis 5 strains, Fusarium 19 strains, Neonectria 2 strains, Chaetomium 4 strains, Calonectria 5 strains, Xylaria 5 strains, Paecilomyces 7 strains, Alternaria 2 strains, Phomopsis 8 strains, Colletotrichum 32 strains, Rhizopus 5 strains, Mucor 7 strains; Albugo 6 strains. Acorrding to the ITS sequences, another 10 strains needed more identification, which had low degree of similarity with Botryosphaeria sp., Beltraniella sp.. It showed that Colletotrichum sp., Penicillium sp., Fusarium sp. were the predominant species, and had the richest biodiversity in tropical coniferous forests. |
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