| 石广举，孙力军，王雅玲，刘唤明，徐德峰，聂芳红，刘颖，叶日英.纳豆芽孢杆菌NT-6 抗菌脂肽对凡纳滨对虾生长性能及非特异性免疫指标的影响[J].广东农业科学,2014,41(22):99-103 |
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纳豆芽孢杆菌NT-6 抗菌脂肽对凡纳滨对虾生长性能及非特异性免疫指标的影响 |
| Effects of antibacterial lipopeptides produced by Bacillus subtilis NT-6 in solid-state fermentation on growth performance and non-specific immunity of Litopenaeus vannamei |
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| DOI： |
| 中文关键词: 抗菌脂肽 凡纳滨对虾 生长 非特异性免疫 |
| 英文关键词: antibacterial lipopeptides Litopenaeus vannamei growth non-specific immunity |
| 基金项目:国家自然科学基金（30972287）；国家“十二五”科技支撑计划项目（2012BAD29B06）；广东省海洋战略性新兴产业专项成果转化及产业化项目（GD2012-B02-007）；湛江市科技计划项目（2011D02） |
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| 中文摘要: |
| 为了评价纳豆菌抗菌脂肽对凡纳滨对虾（Litopenaeus vannamei）生长和非特异免疫能力的影响，在基础组饲料中分别添加0mg/kg（A0组，对照组）、50mg/kg（A1组）、100 mg/kg（A2组）、150 mg/kg（A3 组）和200 mg/kg（A4组）NT-6 抗菌脂肽，分别投喂平均体重为1.12（±0.10）g的凡纳滨对虾8周，测量和计算对虾的生长和形态指标以及对虾血清和肝胰腺中碱性磷酸酶（AKP）、超氧化物歧化酶（SOD）、溶菌酶（LZM）、总抗氧化能力（T-AOC）及血清中过氧化物酶（POD）活力。结果显示，经过8周的喂养，试验组对虾生长速度均比对照组快，其中A2组对虾的生长速度最快，相对增重率可达58.13%；试验组对虾的各形态指标受抗菌脂肽添加量影响显著，对虾的整体外观商品形态有所改善。试验组对虾肌肉中粗蛋白、脂肪的含量与对照组相比差异显著，粗灰分差异不显著。试验组凡纳滨对虾血清中LZM活性均高于对照组，其中A2组最显著；A1组血清中的POD 活性高于其他各组；各组血清中的AKP 活性差异不显著；试验组凡纳滨对虾血清中T-AOC和T-SOD 活性与对照组相比差异显著且A2 组最高。A4组凡纳滨肝胰腺中T-AOC、LZM、AKP、T-SOD活性明显高于对照组。对虾非特异性免疫指标的提高使对虾的成活率得到了一定的提高，其中A2组的成活率最高。结果表明，在饲料中添加一定量的NT-6抗菌脂肽可显著促进凡纳滨对虾的生长和提高对虾机体的非特异性免疫能力，可作为抗生素饲料添加剂的一种替代物。 |
| 英文摘要: |
| Bacillus natto antimicrobial lipopeptide is a new type of antibacterial peptides of microorganisms found in recent years, with potential to be developed into a green feed additive, the purpose of this study is to evaluate its effect ongrowth promoting and non-specific immunity of Litopenaeus vannamei. Added 0 mg/kg, 50 mg/kg, 100 mg/kg, 150 mg/kg and 200 mg/kg antibacterial lipopeptide in basic diet respectively to feed L. vannamei of group A0, A1, A2, A3 and A4 which was 1.12 (±0.10) g in weight for 8 weeks, and AKP (alkaline phosphatase), T-SOD (superoxide dismutase), LZM(lysozyme), T -AOC (total antioxidant capacity) in serum and hepatopancreas and POD (peroxidase ) in serum andmorphological indexes of shrimp were measured. Results showed that after 8 weeks of feeding experiment, the growth rate of shrimp of experiment groups was faster than that of control group, in which the shrimp of group A2 was the fastest and its relative growth rate reached 58.13%. The morphological indexes of shrimp of experimental groups
were affected by antimicrobial lipopeptides significantly,and the overall appearance of the commodity form was improved. The content of crude protein and fat in muscle of experimental groups had significant difference compared with those of control group, but the difference of crude ash was not significant. The activity of LZM in serum of experimental groups was significantly higher than that in control group, and the group A2 was affected most significantly. The activity of POD in serum of group A1 was higher than that of other groups. The difference of AKP activity in the serum
of the experimental groups was not significant. T-AOC and T-SOD activity in serum of experimental groups had significant differences compared with control group, and the group A2 had the highest. The activity of T-AOC, LZM,AKP and T -SOD of hepatopancreas in group A4 was significantly higher than those in control group. The improvement of non-specific immune indexes of shrimp contributed to improving its survival rate to some extent, and the survival rate of group A2 was the highest. The result indicated that adding a certain amount of NT -6antimicrobial lipopeptides in the feed could significantly promote the growth of L. vannamei and improve its nonspecific immunity, and therefore the antimicrobial lipopeptides could be used as a substitute of antibiotic feed additives. |
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